RHBDF2 Rabbit Polyclonal Antibody

Scheda tecnica

Scheda di sicurezza

Key features and details

Rabbit polyclonal antibody to RHBDF2

Obiettivo: RHBDF2
Fonte/Ospite: Coniglio
Reattività: Umano, topo
Clonalità: Policlonale
Applicazioni: WB
Coniugazione: Non coniugato
Stoccaggio: a-20°C

Marca:

CAT.NO. : APA17998

US$ Please choose

Dimensioni:

50μL 100μL

Trail, Bulk size or Custom requests Please contact us

Dettagli del prodotto

Sfondo

Regulates ADAM17 protease, a sheddase of the epidermal growth factor (EGF) receptor ligands and TNF, thereby plays a role in sleep, cell survival, proliferation, migration and inflammation. Does not exhibit any protease activity on its own.

Applicazione

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:500 - 1:1000

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Panoramica

Descrizione	Rabbit polyclonal antibody to RHBDF2
Specificità	Recognizes endogenous levels of RHBDF2 protein.
Tipo di anticorpo	Primary antibody
Immunogeno	KLH-conjugated synthetic peptide encompassing a sequence within the N-terminal region of human RHBDF2. The exact sequence is proprietary.
Purificazione	The antibody was purified by immunogen affinity chromatography.
Molecular Weight	Predicted: 96 kD; Observed: 110 kD
Modulo/Buffer	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative Names	IRHOM2; RHBDL5; RHBDL6; Inactive rhomboid protein 2; iRhom2; Rhomboid 5 homolog 2; Rhomboid family member 2; Rhomboid veinlet-like protein 5; Rhomboid veinlet-like protein 6
Simbolo del gene	RHBDF2
Entrez Gene	79651(Human); 217344(Mouse)
SwissProt	Q6PJF5(Human); Q80WQ6(Mouse)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Dati

Western blot analysis of RHBDF2 expression in mouse lung (A) whole cell lysates. (Predicted band size: 96 kD; Observed band size: 110 kD)

Stoccaggio

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Nota

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

Domande frequenti

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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