PRAMEF20/21 Rabbit Polyclonal Antibody

Scheda tecnica

Scheda di sicurezza

Key features and details

Rabbit polyclonal antibody to PRAMEF20/21

Obiettivo: PRAMEF20/21
Fonte/Ospite: Coniglio
Reattività: Umano, topo, ratto
Clonalità: Policlonale
Applicazioni: WB, IHC
Coniugazione: Non coniugato
Stoccaggio: a-20°C

Marca:

CAT.NO. : APA16578

US$ Scegli

Dimensioni:

50μL 100μL

Trail, Bulk size or Custom requests Please contact us

Dettagli del prodotto

Sfondo

PRAME family member 20 (PRAMEF20). Belongs to the PRAME family

Applicazione

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:500 - 1:2000
IHC	1:50 - 1:200

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Panoramica

Descrizione	Rabbit polyclonal antibody to PRAMEF20/21
Specificità	Recognizes endogenous levels of PRAMEF20/21 protein
Tipo di anticorpi	Anticorpo primario
Immunogeno	Recombinant fusion protein of human PRAMEF20/21. The exact sequence is proprietary.
Purificazione	The antibody was purified by immunogen affinity chromatography.
Peso Molecolare	Predicted: 55 kD; Observed: 55 kD
Modulo/Buffer	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Nomi alternativi	PRAME family member 20/21
Simbolo del gene	PRAMEF20; PRAMEF21
Entrez Gene	391001; 645425(Human); 627009(Mouse)
SwissProt	Q5VT98(Umano)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Dati

Western blot analysis of PRAMEF20/21 expression in mouse heart (A), rat heart (B) whole cell lysates. (Predicted band size: 55 kD; Observed band size: 55 kD)

Immunohistochemical analysis of PRAMEF20/21 staining in mouse brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Stoccaggio

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Nota

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

Domande frequenti

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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