NRBP1 Rabbit Polyclonal Antibody

Key features and details

Rabbit polyclonal antibody to NRBP1
  • Obiettivo: NRBP1
  • Fonte/Ospite: Coniglio
  • Reattività: Human,Mouse,Rat,Monkey
  • Clonalità: Policlonale
  • Applicazioni: WB
  • Coniugazione: Non coniugato
  • Stoccaggio: a-20°C
  • Marca:
N. CAT. : APA10287
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Dimensioni:
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Dettagli del prodotto
Sfondo
Required for embryonic development . Plays a role in intestinal epithelial cell fate and proliferation, thereby involved in the architectural development of the intestine potentially via the regulation of Wnt-responsive genes . May play a role in subcellular trafficking between the endoplasmic reticulum and Golgi apparatus through interactions with the Rho-type GTPases . Binding to the NS3 protein of dengue virus type 2 appears to subvert this activity into the alteration of the intracellular membrane structure associated with flaviviral replication .
Applicazione
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB

1:500 - 1:1000

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
Panoramica

Descrizione

Rabbit polyclonal antibody to NRBP1

Specificità

Recognizes endogenous levels of NRBP1 protein.

Tipo di anticorpi

Anticorpo primario

Immunogeno

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human NRBP1. The exact sequence is proprietary.

Purificazione

The antibody was purified by immunogen affinity chromatography.

Peso Molecolare

Predicted: 59 kD; Observed: 74 kD

Modulo/Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Nomi alternativi

BCON3; NRBP; Nuclear receptor-binding protein

Simbolo del gene

NRBP1

Entrez Gene

29959(Human); 192292(Mouse)

SwissProt

Q9UHY1(Human); Q99J45(Mouse)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Dati

Western blot analysis of NRBP1 expression in A549 (A), PC12 (B), CT26 (C) whole cell lysates. (Predicted band size: 59 kD; Observed band size: 74 kD)

Stoccaggio
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Nota
For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.
Domande frequenti
What are the main types of research antibodies and how do they differ?
Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.
How can I tell if a research antibody is suitable for my experiment?
It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.
Can improper storage of research antibodies affect experimental results?
Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.
Why doesn’t the recommended dilution in the datasheet work well in my experiment?
The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.
What precautions should I take when using a newly purchased research antibody for the first time?
It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.
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