Anticorpo policlonale di coniglio LRRK2

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Anticorpo policlonale di coniglio contro LRRK2

Obiettivo: LRRK2
Fonte/Ospite: Coniglio
Reattività: Umano, topo, ratto
Clonalità: Policlonale
Applicazioni: WB, IHC, IF/ICC
Coniugazione: Non coniugato
Stoccaggio: a-20°C

Marca:

N. CAT. : APA17412

US$ Scegli

Dimensioni:

50μL 100μL

Trail, dimensioni sfuse o richieste personalizzate Vi preghiamo di contattarci

Dettagli del prodotto

Sfondo

Serine/threonine-protein kinase which phosphorylates a broad range of proteins involved in multiple processes such as neuronal plasticity, innate immunity, autophagy, and vesicle trafficking . Is a key regulator of RAB GTPases by regulating the GTP/GDP exchange and interaction partners of RABs through phosphorylation . Phosphorylates RAB3A, RAB3B, RAB3C, RAB3D, RAB5A, RAB5B, RAB5C, RAB8A, RAB8B, RAB10, RAB12, RAB29, RAB35, and RAB43 . Regulates the RAB3IP-catalyzed GDP/GTP exchange for RAB8A through the phosphorylation of 'Thr-72' on RAB8A . Inhibits the interaction between RAB8A and GDI1 and/or GDI2 by phosphorylating 'Thr-72' on RAB8A . Regulates primary ciliogenesis through phosphorylation of RAB8A and RAB10, which promotes SHH signaling in the brain .

Applicazione

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:500 - 1:1000
IHC	1:50 - 1:200
SE/ICC	1:50 - 1:200

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Panoramica

Descrizione	Anticorpo policlonale di coniglio contro LRRK2
Specificità	Riconosce i livelli endogeni della proteina LRRK2.
Tipo di anticorpo	Anticorpo primario
Immunogeno	Recombinant fusion protein of human LRRK2. The exact sequence is proprietary.
Purificazione	L'anticorpo è stato purificato mediante cromatografia di affinità immunogena.
Peso Molecolare	Previsto: 286 kD; Osservato: 286 kD
Modulo/Buffer	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Nomi alternativi	PARK8; Leucine-rich repeat serine/threonine-protein kinase 2; Dardarin
Simbolo del gene	LRRK2
Entrez Gene	120892(Umano); 66725(Topo)
SwissProt	Q5S007(Umano); Q5S006(mouse)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Dati

Western blot analysis of LRRK2 expression in HepG2 (A), mouse lung (B) whole cell lysates. (Predicted band size: 286 kD; Observed band size: 286 kD)

Immunohistochemical analysis of LRRK2 staining in mouse kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of LRRK2 staining in C2C12 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Stoccaggio

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Nota

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

Domande frequenti

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

Come posso sapere se un anticorpo di ricerca è adatto al mio esperimento?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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