Anticorpo policlonale di coniglio EMP

Scheda tecnica

Scheda di sicurezza

Caratteristiche principali e dettagli

Anticorpo policlonale di coniglio anti-EMP

Obiettivo: EMP
Fonte/Ospite: Coniglio
Reattività: Umano, topo, ratto, bovino, pollo, scimmia
Clonalità: Policlonale
Applicazioni: WB, IHC, IF/ICC
Coniugazione: Non coniugato
Stoccaggio: a-20°C

Marca:

N. CAT. : APA08759

US$ Scegli

Dimensioni:

50μL 100μL

Trail, dimensioni sfuse o richieste personalizzate Vi preghiamo di contattarci

Dettagli del prodotto

Sfondo

Core component of the CTLH E3 ubiquitin-protein ligase complex that selectively accepts ubiquitin from UBE2H and mediates ubiquitination and subsequent proteasomal degradation of the transcription factor HBP1. MAEA and RMND5A are both required for catalytic activity of the CTLH E3 ubiquitin-protein ligase complex . MAEA is required for normal cell proliferation . The CTLH E3 ubiquitin-protein ligase complex is not required for the degradation of enzymes involved in gluconeogenesis, such as FBP1 . Plays a role in erythroblast enucleation during erythrocyte maturation and in the development of mature macrophages . Mediates the attachment of erythroid cell to mature macrophages; this MAEA-mediated contact inhibits erythroid cell apoptosis .

Applicazione

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:500 - 1:1000
IHC	1:100 - 1:200
SE/ICC	1:50 - 1:200

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Panoramica

Descrizione	Anticorpo policlonale di coniglio anti-EMP
Specificità	Riconosce i livelli endogeni della proteina EMP.
Tipo di anticorpi	Anticorpo primario
Immunogeno	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human EMP. The exact sequence is proprietary.
Purificazione	L'anticorpo è stato purificato mediante cromatografia di affinità immunogena.
Peso Molecolare	Previsto: 45 kD; Osservato: 45 kD
Modulo/Buffer	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Nomi alternativi	EMP; Macrophage erythroblast attacher; Cell proliferation-inducing gene 5 protein; Erythroblast macrophage protein; Human lung cancer oncogene 10 protein; HLC-10
Simbolo del gene	MAEA
Entrez Gene	10296(Umano); 59003(Topo); 298982(Ratto)
SwissProt	Q7L5Y9(Umano); Q4VC33(mouse); Q5RKJ1(Ratto)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Dati

Western blot analysis of EMP expression in HepG2 (A), HeLa (B) whole cell lysates. (Predicted band size: 45 kD; Observed band size: 45 kD)

Immunohistochemical analysis of EMP staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of EMP staining in HEPG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AREX® Fluor 594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Stoccaggio

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Nota

Solo per uso di ricerca. Non per uso diagnostico, terapeutico, profilattico o in vivo.

Domande frequenti

Quali sono i principali tipi di anticorpi di ricerca e in cosa differiscono?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

Come posso sapere se un anticorpo di ricerca è adatto al mio esperimento?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

La conservazione impropria degli anticorpi di ricerca può influenzare i risultati sperimentali?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Perché la diluizione consigliata nella scheda tecnica non funziona bene nel mio esperimento?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

Nuovi prodotti

Anticorpo monoclonale murino TRBC1 (ARA662)

Topo monoclonale a TRBC1

Anticorpo monoclonale murino OCT2 (C2325)

Anticorpo monoclonale murino contro OCT2

Buffer DAB ARExVisual®

Diluente dell'anticorpo primario

Cromogeno ARexVisual® DAB (20×)

Anticorpo monoclonale di coniglio CD68 (ARA633)