Anticorpo policlonale di coniglio DNA-PKcs (Phospho-T2647).

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Scheda di sicurezza

DNA-PKcs (Phospho-T2647) Rabbit Polyclonal Antibody

Caratteristiche principali e dettagli

Anticorpo policlonale di coniglio contro DNA-PKcs (Phospho-T2647)

Obiettivo: DNA-PKcs (Fosfo-T2647)
Fonte/Ospite: Coniglio
Reattività: Umano, Scimmia
Clonalità: Policlonale
Applicazioni: WB, IHC, IF/ICC
Coniugazione: Non coniugato
Stoccaggio: a-20°C

Marca:

N. CAT. : APA10014

US$ Scegli

Dimensioni:

50μL 100μL

Trail, dimensioni sfuse o richieste personalizzate Vi preghiamo di contattarci

Dettagli del prodotto

Sfondo

Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage . Involved in DNA non-homologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination . Must be bound to DNA to express its catalytic properties . Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C) . Recruited by XRCC5 and XRCC6 to DNA ends and is required to (1) protect and align broken ends of DNA, thereby preventing their degradation, (2) and sequester the DSB for repair by NHEJ . Acts as a scaffold protein to aid the localization of DNA repair proteins to the site of damage . The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step .

Applicazione

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:500 - 1:1000
IHC	1:100 - 1:200
SE/ICC	1:100 - 1:500

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Panoramica

Descrizione	Anticorpo policlonale di coniglio contro DNA-PKcs (Phospho-T2647)
Specificità	Riconosce i livelli endogeni della proteina DNA-PKcs solo quando fosforilata a T2647.
Tipo di anticorpo	Anticorpo primario
Immunogeno	KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T2647 of human DNA-PKcs protein. The exact sequence is proprietary.
Purificazione	L'anticorpo è stato purificato mediante cromatografia di affinità immunogena.
Peso Molecolare	Previsto: 469 kD; Osservato: 460 kD
Modulo/Buffer	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Nomi alternativi	HYRC; HYRC1; DNA-dependent protein kinase catalytic subunit; DNA-PK catalytic subunit; DNA-PKcs; DNPK1; p460
Simbolo del gene	PRKDC
Entrez Gene	5591(Umano)
SwissProt	P78527(Umano)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Dati

Western blot analysis of DNA-PKcs (Phospho-T2647) expression in Jurkat (A), HeLa (B) whole cell lysates. (Predicted band size: 469 kD; Observed band size: 460 kD)

Immunohistochemical analysis of DNA-PKcs (Phospho-T2647) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of DNA-PKcs (Phospho-T2647) staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Stoccaggio

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Nota

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

Domande frequenti

Quali sono i principali tipi di anticorpi di ricerca e in cosa differiscono?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

Come posso sapere se un anticorpo di ricerca è adatto al mio esperimento?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

La conservazione impropria degli anticorpi di ricerca può influenzare i risultati sperimentali?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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