CD45 (Phospho-Y1216) Rabbit Polyclonal Antibody

Scheda tecnica

Scheda di sicurezza

CD45 (Phospho-Y1216) Rabbit Polyclonal Antibody

Caratteristiche principali e dettagli

Rabbit polyclonal antibody to CD45 (Phospho-Y1216)

Obiettivo: CD45 (fosforo-Y1216)
Fonte/Ospite: Coniglio
Reattività: Umano, topo, ratto
Clonalità: Policlonale
Applicazioni: WB
Coniugazione: Non coniugato
Stoccaggio: a-20°C

Marca:

N. CAT. : APA10693

US$ Scegli

Dimensioni:

50μL 100μL

Trail, Bulk size or Custom requests Please contact us

Dettagli del prodotto

Sfondo

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor . Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity . Interacts with CLEC10A at antigen presenting cell-T cell contact; CLEC10A on immature dendritic cells recognizes Tn antigen-carrying PTPRC/CD45 receptor on effector T cells and modulates T cell activation threshold to limit autoreactivity.

Applicazione

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:500 - 1:1000

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Panoramica

Descrizione	Rabbit polyclonal antibody to CD45 (Phospho-Y1216)
Specificità	Recognizes endogenous levels of CD45 protein only when phosphorylated at Y1216.
Tipo di anticorpi	Anticorpo primario
Immunogeno	KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y1216 of human CD45 protein. The exact sequence is proprietary.
Purificazione	The antibody was purified by immunogen affinity chromatography.
Peso Molecolare	Previsto: 147 kD; Osservato: 180 kD
Modulo/Buffer	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Nomi alternativi	CD45; Receptor-type tyrosine-protein phosphatase C; Leukocyte common antigen; L-CA; T200; CD45
Simbolo del gene	PTPRC
Entrez Gene	5788(Umano); 24699(Ratto)
SwissProt	P08575(Human); P06800(Mouse); P04157(Rat)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Dati

Western blot analysis of CD45 (Phospho-Y1216) expression in HEK293T (A), MCF7 (B), PC12 (C), AML12 (D) whole cell lysates. (Predicted band size: 147 kD; Observed band size: 180 kD)

Direct ELISA antibody dose-response curve using Anti-CD45 (Phospho-Y1216) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.

Stoccaggio

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Nota

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

Domande frequenti

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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