Tag HA Antibodi Monoklonal Kelinci (ARA782)

Lembar Data

MSDS

HA tag Rabbit Monoclonal Antibody(ARA782)

Fitur dan detail utama

Sasaran: tanda HA
Sumber/Pembawa Acara: Kelinci
Reaktivitas: Spesies independen
Klonalitas: Monoklonal
Aplikasi: WB, JIKA/ICC, FC, IP
Konjugasi: Tidak terkonjugasi
Penyimpanan: pada-20°C

Merek:

KUCING.TIDAK. : ARA6569

US$ Silakan pilih

Ukuran:

100μL

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Detail Produk

Latar Belakang

HA tag Recombinant Rabbit Monoclonal Antibody Product Datasheet Predicted Molecular Wt: Depending on sample to be analyzedkDa Purity: ProA affinity purified IgG Species independent Species Cross-reactivity: Form: Liquid Species cross-reactivity determined by WB Swissprot ID: N/A Applications: WB IF/ICC FC IP Background: Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag derived from the HA molecule corresponding to amino acids

Aplikasi

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:1000-1:2000
JIKA/ICC	1:400-1:2000
FC	1:400-1:2000
IP	1:10

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Ikhtisar

Deskripsi	Antibodi Monoklonal Kelinci terhadap tag HA
Tipe Antibodi	Antibodi primer
Prediksi UM
Imunogen	YPYDVPDYA (influenza hemagglutinin-HA-epitope) terkonjugasi ke KLH.
Pemurnian	IgG murni afinitas ProA
Formulir/Penyangga	PBS 59%, Natrium azida 0,01%, Gliserol 40%, BSA 0,57%.
Nama Alternatif	tag epitop HA; hemaglutinin

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Prediksi UM: Tergantung pada protein fusi dengan tag HA
Lane 1: 293 cells lysate transfected with C-terminal HA tagged gene (ARA782 at 1:2,000 dilution).
Lane 2: 293 cells lysate transfected with N-terminal HA tagged gene (ARA782 at 1:1,000 dilution).
Jalur 3: 293 sel lisat tanpa transfeksi apa pun (ARA782 pada pengenceran 1:400).
Jalur 1: 1 µg per jalur
Jalur 2/3: 10 µg per jalur
Ab ke-2:
GAR HRP(H+L) 1:5.000
Paparan: 20 detik

Overlay histogram showing 293 cells transfected with N-terminal (Green) and C-terminal (Red) HA tagged gene stained with ARA782. The cells were then incubated in the antibody (ARA782, 1:2,000 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody used was a Goat Anti-Rabbit Alexa Fluor^® 488 (IgG H+L) at 1:2,000 dilution for 20 min at room temperature. Unlabelled sample (Black) was used as a control.

ARA782 staining HA tag in 293 cells transfected with N-terminal HA tagged gene by IF/ICC (immunofluorescence/immunocytochemistry). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with primary antibody (1:2,000) at 4°C. An Alexa Fluor^® 594-conjugated Goat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1:500). DAPI (blue) was used as the nuclear counter stain.

Control: PBS and secondary antibody, An Alexa Fluor^® 594-conjugated Goat Anti-Rabbit IgG (1:500).

ARA782 staining HA tag in 293 cells transfected with C-terminal HA tagged gene by IF/ICC (immunofluorescence/immunocytochemistry). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with primary antibody (1:2,000) at 4°C. An Alexa Fluor^® 594-conjugated Goat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1:500). DAPI (blue) was used as the nuclear counter stain.

Control: PBS and secondary antibody, An Alexa Fluor^® 594-conjugated Goat Anti-Rabbit IgG (1:500).

HA tag was immunoprecipitated from 0.2mg of 293 whole cells lysate transfected with N-terminal HA tagged gene with ARA782 at 1:10 dilution.
Ab ke-2:
GAR HRP untuk IP 1:500

Jalur 1: IP ARA782 dalam 293 lisat sel utuh ditransfeksi dengan gen bertanda N-terminal HA
Lane 2: PBS instead of ARA782 in 293 whole cell lysate transfected with N-terminal HA tagged gene
Jalur 3: 293 lisat sel utuh ditransfeksi dengan gen bertanda N-terminal HA, 10 µg (input)

Paparan: 60an

HA tag was immunoprecipitated from 0.2mg of 293 whole cells lysate transfected with C-terminal HA tagged gene with ARA782 at 1:10 dilution.
Ab ke-2:
GAR HRP untuk IP 1:500

Jalur 1: IP ARA782 dalam 293 lisat sel utuh ditransfeksi dengan gen bertanda C-terminal HA
Jalur 2: PBS sebagai pengganti ARA782 pada 293 lisat sel utuh ditransfeksi dengan gen bertanda C-terminal HA
Jalur 3: 293 lisat sel utuh ditransfeksi dengan gen bertanda C-terminal HA, 10 µg (input)

Paparan: 60an

Penyimpanan

Simpan pada suhu 4°C untuk jangka pendek. Untuk penyimpanan jangka panjang, simpan pada suhu -20°C, hindari siklus pembekuan/pencairan.

Catatan

Hanya Untuk Penggunaan Penelitian. Bukan untuk penggunaan diagnostik, terapeutik, profilaksis, atau in vivo.

FAQ

Apa jenis utama antibodi penelitian dan apa perbedaannya?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

Bagaimana saya dapat mengetahui apakah antibodi penelitian cocok untuk eksperimen saya?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Apakah penyimpanan antibodi penelitian yang tidak tepat dapat mempengaruhi hasil eksperimen?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Mengapa pengenceran yang disarankan dalam lembar data tidak berfungsi dengan baik dalam percobaan saya?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

Tindakan pencegahan apa yang harus saya ambil ketika menggunakan antibodi penelitian yang baru dibeli untuk pertama kalinya?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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