Human Granzyme A/GZMA ELISA Kit
Protocol Booklet 
Key features and details
- Species:
- Size:
- Sample Volume:
- Standard Curve Range:
- Assay type:
- Sensitivity:
- Operation Duration:
- Assay type:
-
Brand:
CAT.NO. : AEH0129
US$ Please choose
US$ Please choose
Size:
Trail, Bulk size or Custom requests Please contact us
Product Details
Background
Granzyme family serine proteases are stored in the granules of cytotoxic T lymphocytes and natural killer cells. Granzymes contain one S1 protease domain and are synthesized as preproproteins. Granzymes are released toward pathogen infected or transformed target cells during cellular immune reactions. Target cell entry by granzymes is through perforin channels. Granzymes trigger apoptosis by caspase-dependent and -independent mechanisms. There are five granzymes (A, B, G, H and K) in human and many more in mouse and rat.
typical data
|
pg/ml |
O.D. |
Average |
Corrected |
|
|
0.00 |
0.0136 |
0.0141 |
0.0139 |
|
|
2.74 |
0.0255 |
0.0239 |
0.0247 |
0.0109 |
|
8.23 |
0.0392 |
0.0410 |
0.0401 |
0.0263 |
|
24.69 |
0.0845 |
0.0824 |
0.0835 |
0.0696 |
|
74.07 |
0.2141 |
0.2112 |
0.2127 |
0.1988 |
|
222.22 |
0.6253 |
0.5911 |
0.6082 |
0.5944 |
|
666.67 |
1.7910 |
1.7440 |
1.7675 |
1.7537 |
|
2000.00 |
3.7830 |
3.7900 |
3.7865 |
3.7727 |
precision
|
Intra-assay Precision |
Inter-assay Precision |
|||||
|
Sample Number |
S1 |
S2 |
S3 |
S1 |
S2 |
S3 |
|
22 |
22 |
22 |
6 |
6 |
6 |
|
|
Average（pg/ml） |
49.6 |
198.7 |
609.8 |
47.5 |
189.0 |
608.9 |
|
Standard deviation |
2.3 |
8.7 |
26.3 |
1.5 |
7.3 |
20.0 |
|
Coefficient of variation（%） |
4.6 |
4.4 |
4.3 |
3.2 |
3.9 |
3.3 |
- Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
- Inter-assay Precision (Precision between assays)Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
spike recovery
The spike recovery was evaluated by spiking 3 levels of human Granzyme A into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 81% to 97% with an overall mean recovery of 91%.
The recovery ranged from 81% to 97% with an overall mean recovery of 91%.
sample values
|
Sample Matrix |
Sample Evaluated |
Range (pg/ml) |
Detectable % |
Mean of Detectable (pg/ml) |
|
Serum |
30 |
62.47-179.55 |
100.0% |
113.58 |
New Products
