Human CD276 ARExSet® antibody pair for ELISA
CAT.NO. : ASEH0101
US$ Please choose
US$ Please choose
Typical data
|
pg/ml |
O.D. |
Average |
Corrected |
|
|
0.00 |
0.0181 |
0.0189 |
0.0185 |
|
|
13.72 |
0.0808 |
0.0821 |
0.0815 |
0.0630 |
|
41.15 |
0.1912 |
0.1890 |
0.1901 |
0.1716 |
|
123.46 |
0.4793 |
0.5034 |
0.4914 |
0.4729 |
|
370.37 |
1.0330 |
1.1070 |
1.0700 |
1.0515 |
|
1111.11 |
2.1320 |
2.1260 |
2.1290 |
2.1105 |
|
3333.33 |
3.1930 |
3.1940 |
3.1935 |
3.1750 |
|
10000.00 |
3.7990 |
3.7856 |
3.7923 |
3.7738 |
Product Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Economical alternative to complete kits
Kit Content
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
ARExSet® Ancillary Reagent Kit (5 plates): containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and assay buffer.
- 96 well microplates: AREX Biosciences, Catalog # DSEP01. Plate Sealers: AREX Biosciences, Catalog # DSSF01.
- Coating Buffer: 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2-7.4, 0.2 μm fi
ltered . AREX Biosciences, Catalog # DSCB01. - Blocking Buffer: AREX Biosciences, Catalog # DSBB01.
- Wash Buffer: 0.05% Tween 20 in PBS, pH 7.2-7.4. AREX Biosciences, Catalog # DSWB01.
- Assay Buffer: 0.5%BSA,0.05%Tween20,PBS Solution. AREX Biosciences, Catalog # DSAB01
Substrate Solution: Tetramethylbenzidine. AREX Biosciences, Catalog # DSTS01. - Stop Solution: 0.5mol/ml H2SO4. AREX Biosciences, Catalog # DSSS01.
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