Human CD276 ARExSet® antibody pair for ELISA

Key features and details

  • Species: Human
  • Size: 5 plates,15 plates,50 plates
  • Sample Volume: 50 μl
  • Standard Curve Range: 13.72 pg/ml-10000 pg/ml
  • Assay type: Not Ready-to-Use
  • Sensitivity: 0.12pg/ml(50 μl)
  • Operation Duration: 120min
  • Assay type: Sandwich Elisa
  • Brand:
CAT.NO. : ASEH0101
US$ Please choose
US$ Please choose
Trail, Bulk size or Custom requests Please contact us
Product Details
Typical data


pg/ml

O.D.

Average

Corrected

0.00

0.0181

0.0189

0.0185

13.72

0.0808

0.0821

0.0815

0.0630

41.15

0.1912

0.1890

0.1901

0.1716

123.46

0.4793

0.5034

0.4914

0.4729

370.37

1.0330

1.1070

1.0700

1.0515

1111.11

2.1320

2.1260

2.1290

2.1105

3333.33

3.1930

3.1940

3.1935

3.1750

10000.00

3.7990

3.7856

3.7923

3.7738

Product Features
  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
Assay can be customized to your specific needs
  • Economical alternative to complete kits
Kit Content
  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
ARExSet® Ancillary Reagent Kit (5 plates): containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and assay buffer.
  1. 96 well microplates: AREX Biosciences, Catalog # DSEP01. Plate Sealers: AREX Biosciences, Catalog # DSSF01.
  2. Coating Buffer: 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2-7.4, 0.2 μm fi
    ltered . AREX Biosciences, Catalog # DSCB01.
  3. Blocking Buffer: AREX Biosciences, Catalog # DSBB01.
  4. Wash Buffer: 0.05% Tween 20 in PBS, pH 7.2-7.4. AREX Biosciences, Catalog # DSWB01.
  5. Assay Buffer: 0.5%BSA,0.05%Tween20,PBS Solution. AREX Biosciences, Catalog # DSAB01
    Substrate Solution: Tetramethylbenzidine. AREX Biosciences, Catalog # DSTS01.
  6. Stop Solution: 0.5mol/ml H2SO4. AREX Biosciences, Catalog # DSSS01. 
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