HSPA4 Mouse Monoclonal Antibody(C2751)

Datasheet

MSDS

Key features and details

Mouse monoclonal to HSPA4

Target: HSPA4
Source/Host: Mouse
Reactivity: Human,Mouse,Rat,Monkey
Clonality: Monoclonal
Applications: WB, IHC, FC
Conjugation: Unconjugated
Storage: at-20°C

Brand:

CAT.NO. : AMA02363

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Size:

50μL 100μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

Heat shock 70 kDa protein 4 (HSPA4), also known as HSP70RY, Heat shock 70-related protein APG-2, Heat shock protein family H member 2. Belongs to the heat shock protein 70 family Subunit: Interacts with TJP1/ZO-1 Subcellular location: Cytoplasm

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:500 - 1:1000
IHC	1:100 - 1:500
FC	1:100 - 1:200

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Description	Mouse monoclonal to HSPA4
Specificity	Recognizes endogenous levels of HSPA4 protein
Antibody Type	Primary antibody
Imnunogen	Recombinant fusion protein of human HSPA4 expressed in HEK293
Purification	This antibody is purified through a protein G column.
Molecular Weight	Predicted: 94 kD; Observed: 100 kD kD
Form/Buffer	Mouse IgG2b. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative Names	APG2; Heat shock 70 kDa protein 4; HSP70RY; Heat shock 70-related protein APG-2
Gene Symbol	HSPA4
Entrez Gene	3308(Human); 266759(Rat)
SwissProt	P34932(Human); Q61316(Mouse); O88600(Rat)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Western blot analysis of HSPA4 expression in Hela (A), HepG2 (B), HEK293 (C), PC12 (D), A549 (E), Jurkat (F), COS7 (G), NIH/3T3 (H) whole cell lysates. (Predicted band size: 94 kD; Observed band size: 100 kD kD)

Immunohistochemical analysis of HSPA4 staining in human bladder cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Flow cytometric analysis of HeLa cells using Anti-HSPA4 Antibody (green) and negative control (red).

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Note

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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