HLA-F Mouse Monoclonal Antibody(C2746)
Datasheet 
Key features and details
Mouse monoclonal to HLA-F
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
CAT.NO. : AMA02358
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Product Details
Background
Non-classical major histocompatibility class Ib molecule postulated to play a role in immune surveillance, immune tolerance and inflammation. Functions in two forms, as a heterotrimeric complex with B2M/beta-2 microglobulin and a peptide (peptide-bound HLA-F-B2M) and as an open conformer (OC) devoid of peptide and B2M (peptide-free OC). In complex with B2M, presents non-canonical self-peptides carrying post-translational modifications, particularly phosphorylated self-peptides. Peptide-bound HLA-F-B2M acts as a ligand for LILRB1 inhibitory receptor, a major player in maternal-fetal tolerance. Peptide-free OC acts as a ligand for KIR3DS1 and KIR3DL2 receptors and as an open conformer (OC) devoid of peptide and B2M (peptide-free OC).
Application
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
WB | 1:500 - 1:1000 |
FC | 1:100 - 1:200 |
Overview
Description | Mouse monoclonal to HLA-F |
Specificity | Recognizes endogenous levels of HLA-F protein |
Antibody Type | Primary antibody |
Imnunogen | Recombinant fusion protein of human HLA-F expressed in E. Coli |
Purification | This antibody is purified through a protein G column. |
Molecular Weight | Predicted: 39 kD; Observed: 46 kD kD |
Form/Buffer | Mouse IgG2b. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Names | HLA-5.4; HLAF; HLA class I histocompatibility antigen, alpha chain F; CDA12; HLA F antigen; Leukocyte antigen F; MHC class I antigen F |
Gene Symbol | HLA-F |
Entrez Gene | 3134(Human) |
SwissProt | P30511(Human) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Data
Western blot analysis of HLA-F expression in mouse liver (A), rat liver (B) whole cell lysates. (Predicted band size: 39 kD; Observed band size: 46 kD kD)
Flow cytometric analysis of THP1 cells using Anti-HLA-F Antibody (green) and negative control (red).
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Note
For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.
FAQs
What are the main types of research antibodies and how do they differ?
Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.
How can I tell if a research antibody is suitable for my experiment?
It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.
Can improper storage of research antibodies affect experimental results?
Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.
Why doesn’t the recommended dilution in the datasheet work well in my experiment?
The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.
What precautions should I take when using a newly purchased research antibody for the first time?
It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.
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