Histone H1 (Phospho-T17) Rabbit Monoclonal Antibody(C1556)

Datasheet

MSDS

Histone H1 (Phospho-T17) Rabbit Monoclonal Antibody(C1556)

Key features and details

Recombinant rabbit monoclonal antibody to Histone H1 (Phospho-T17)

Target: Histone H1 (Phospho-T17)
Source/Host: Rabbit
Reactivity: Human
Clonality: Monoclonal
Applications: WB
Conjugation: Unconjugated
Storage: at-20°C

Brand:

CAT.NO. : AMA01168

US$ Please choose

Size:

50μL 100μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber . Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers and promote formation of the H3K27me3 mark by the PRC2/EED-EZH2 complex . Ability to associate with nucleosomes and compact chromatin depends on linker DNA length and trajectory . Also acts as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation .

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:500 - 1:1000

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Description	Recombinant rabbit monoclonal antibody to Histone H1 (Phospho-T17)
Specificity	Recognizes endogenous levels of Histone H1 protein only when phosphorylated at T17
Antibody Type	Primary antibody, Recombinant
Imnunogen	KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T17 of human Histone H1. The exact sequence is proprietary.
Purification	The antibody was purified by immunogen affinity chromatography.
Molecular Weight	Predicted: 21 kD; Observed: 30 kD
Form/Buffer	Liquid in PBS, pH 7.4, containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.
Alternative Names	H1F4; Histone H1.4; Histone H1b; Histone H1s-4
Gene Symbol	HIST1H1E
Entrez Gene	3008(Human)
SwissProt	P10412(Human)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Western blot analysis of Histone H1 (Phospho-T17) expression in K562 (A), THP1 (B), MCF7 (C) whole cell lysates. (Predicted band size: 21 kD; Observed band size: 30 kD)

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Note

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

New Products

hCG alpha Mouse Monoclonal Antibody(ARA629)

E.coli Residual Total RNA Detection Kit (RT-PCR)

Fibronectin Rabbit Monoclonal Antibody(C6)

Antigen Retrieval Solution II (EDTA)

Antigen Retrieval Solution I (Citrate)

Hyaluronic Acid ELISA Kit