Histone Deacetylase 1 (Acetyl-K220) Rabbit Polyclonal Antibody

Datasheet

MSDS

Histone Deacetylase 1 (Acetyl-K220) Rabbit Polyclonal Antibody

Key features and details

Rabbit polyclonal antibody to Histone Deacetylase 1 (Acetyl-K220)

Target: Histone Deacetylase 1 (Acetyl-K220)
Source/Host: Rabbit
Reactivity: Human,Mouse,Rat,Bovine,Chicken
Clonality: Polyclonal
Applications: WB
Conjugation: Unconjugated
Storage: at-20°C

Brand:

CAT.NO. : APA11389

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Size:

50μL 100μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

Histone deacetylase that catalyzes the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) . Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events . Histone deacetylases act via the formation of large multiprotein complexes . Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin . As part of the SIN3B complex is recruited downstream of the constitutively active genes transcriptional start sites through interaction with histones and mitigates histone acetylation and RNA polymerase II progression within transcribed regions contributing to the regulation of transcription .

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:500 - 1:1000

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Description	Rabbit polyclonal antibody to Histone Deacetylase 1 (Acetyl-K220)
Specificity	Recognizes endogenous levels of Histone Deacetylase 1 protein only when acetylated at K220.
Antibody Type	Primary antibody
Imnunogen	KLH-conjugated synthetic acetylated peptide corresponding to residues surrounding K220 of human Histone Deacetylase 1 protein. The exact sequence is proprietary.
Purification	The antibody was purified by immunogen affinity chromatography.
Molecular Weight	Predicted: 55 kD; Observed: 55 kD
Form/Buffer	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative Names	RPD3L1; Histone deacetylase 1; HD1
Gene Symbol	HDAC1
Entrez Gene	3065(Human); 433759(Mouse); 297893(Rat)
SwissProt	Q13547(Human); O09106(Mouse); Q4QQW4(Rat)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Western blot analysis of Histone Deacetylase 1 (Acetyl-K220) expression in mouse pancreas (A) whole cell lysates. (Predicted band size: 55 kD; Observed band size: 55 kD)

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Note

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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