gremlin-1 Mouse Monoclonal Antibody (C-7)
Datasheet 
Key features and details
- Target:
- Host:
- Reactivity:
- Clonality:
- Application:
- Storage:
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Brand:
CAT.NO. : ARA0117
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Product Details
Background
The gremlin protein family contains antagonists of bone morphogenetic protein (BMP) signaling that are expressed in the neural crest. All family members are secreted proteins that act as BMP antagonists in embryonic explants and are expressed in the proximal airway epithelium of the lung during embryonic development. gremlin - 1 is required for early limb outgrowth and patterning in the FGF4 - SHH feedback loop, while gremlin - 2 binds and blocks the activity of BMP - 2 and BMP - 4. The dorsaling factor gremlin belongs to a novel gene family that includes the head - inducing factor Cerberus and the tumor suppressor DAN. Additionally, secreted gremlin relays the Sonic hedgehog signal from the polarizing region to the apical ectodermal ridge.
Application
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
Application | Dilution Ratio |
WB | 1:100-1:1000 |
IF/ICC | 1:50-1:500 |
ELISA | 1:30-1:3000 |
IP | 1-2 µg/100-500 µg total protein |
Overview
Isotype | Mouse IgG2b kappa light chain |
Target Antigen | gremlin-1 |
Gene Name | GREM1 |
UniProt ID | O60565 (Human), O70326 (Mouse), O35793 (Rat) |
Entrez Gene ID | 26585 (Human), 23892 (Mouse), 25629 (Rat) |
Molecular Weight | 23 kDa |
Gene Aliases | CKTSF1B1, DRM, PIG2, DAND2, IHG-2, Cysteine knot superfamily 1 BMP antagonist 1 |
Immunogen | amino acids 32-61 mapping near the N-terminus of gremlin-1 of human origin |
Form/Buffer | PBS with < 0.1% sodium azide and 0.1% gelatin |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Data
Western blot analysis of gremlin - 1 expression in A549 (A), C2C12 (B), P19 (C), Sol8 (D), F9 (E) and L6 (F) whole cell lysates.
Western blot analysis of gremlin - 1 expression in COLO 320DM (A), A549 (B) and Ramos (C) whole cell lysates.
Storage
Store at 4°C, DO NOT FREEZE. Stable for one year from the date of shipment.
Note
For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.
FAQs
What are the main types of research antibodies and how do they differ?
Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.
How can I tell if a research antibody is suitable for my experiment?
It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.
Can improper storage of research antibodies affect experimental results?
Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.
Why doesn’t the recommended dilution in the datasheet work well in my experiment?
The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.
What precautions should I take when using a newly purchased research antibody for the first time?
It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.
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