Exosome-specific MSC Medium (Phenol red-free)
Protocol Booklet 
Key features and details
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Brand:
CAT.NO. : UR51131
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Product Details
Background
Umibio® Exosome‑specific Mesenchymal Stem Cell (MSC) Medium (Phenol Red‑free) and Supplements is a serum‑free, phenol red‑free, chemically defined medium designed specifically for MSC culture and exosome production. No serum or platelet lysate is required during culture, which reduces interference from exogenous particles and enhances exosome secretion by MSCs.
Phenol red is commonly used in culture media as a pH indicator to monitor the acidity and alkalinity of the culture solution. However, it has certain drawbacks. Studies have shown that phenol red can mimic the effects of steroid hormones, especially estrogen. Therefore, phenol red‑free medium is recommended when working with estrogen‑sensitive cells such as mammary tissue. In addition, phenol red may interfere with sodium‑potassium balance under certain conditions and can affect flow cytometry analysis in cell labeling experiments. This medium is phenol red‑free and avoids these potential impacts.
Phenol red is commonly used in culture media as a pH indicator to monitor the acidity and alkalinity of the culture solution. However, it has certain drawbacks. Studies have shown that phenol red can mimic the effects of steroid hormones, especially estrogen. Therefore, phenol red‑free medium is recommended when working with estrogen‑sensitive cells such as mammary tissue. In addition, phenol red may interfere with sodium‑potassium balance under certain conditions and can affect flow cytometry analysis in cell labeling experiments. This medium is phenol red‑free and avoids these potential impacts.
Product Components
|
Component Name |
UR51130 |
UR51131 |
|
Exosome-Specific Interstitial Stem Cell Medium (Xeno-Free) (2-1) |
50 mL |
500 mL |
|
Exosome-Specific Interstitial Stem Cell Medium Additive (2-2) |
0.5 mL |
5 mL |
2.Contains L-Glutamine and Sodium Bicarbonate.
3.Xeno-free, utilizing pharmaceutical-grade proteins and factors (fewer than 3 types).
Features
1.Free from phenol red interference.
2.Simple to use with stable and reliable results.
3.Broad applicability for exosome production from various mesenchymal stem cells.
For exosome production from MSCs only; not suitable for cell passaging.
2.Simple to use with stable and reliable results.
3.Broad applicability for exosome production from various mesenchymal stem cells.
For exosome production from MSCs only; not suitable for cell passaging.
Advantages of the Product
1.Focus on exosome isolation and the R&D of supporting kits.
2.Backed by hundreds of references for confident use and worry-free purchase.
3.Broad applicability: Suitable for exosome production from various mesenchymal stem cells.
4.Serum-free, xeno-free, and chemically defined.
2.Backed by hundreds of references for confident use and worry-free purchase.
3.Broad applicability: Suitable for exosome production from various mesenchymal stem cells.
4.Serum-free, xeno-free, and chemically defined.
Data
Taking hDPSCs cells as an example, these images show the cell state before (left) and 24 hours after (right) using the exosome-specific medium.
Taking hMSC cells as an example, these images display the cell condition before (left) and 24 hours after (right) using the exosome-specific medium.
Shipping temp:
cold temperature
Storage
Store at 2-8°C; store supplements below -18°C. Prepare fresh before use.
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