Anticuerpo policlonal de conejo SMCHD1

MSDS

Características y detalles clave

Anticuerpo policlonal de conejo contra SMCHD1

Objetivo: SMCHD1
Fuente/Anfitrión: Conejo
Reactividad: Humano, Ratón, Rata
Clonalidad: policlonal
Aplicaciones: BM, FI/CCI
Conjugación: Sin conjugar
Almacenamiento: a-20°C

Marca:

CAT.NO. : APA13066

US$ Por favor elige

Tamaño:

50μL 100μL

Trail, Bulk size or Custom requests Please contact us

Detalles del producto

Antecedentes

Non-canonical member of the structural maintenance of chromosomes (SMC) protein family that plays a key role in epigenetic silencing by regulating chromatin architecture . Promotes heterochromatin formation in both autosomes and chromosome X, probably by mediating the merge of chromatin compartments . Plays a key role in chromosome X inactivation in females by promoting the spreading of heterochromatin protein family that plays a key role in epigenetic silencing by regulating chromatin architecture . Promotes heterochromatin formation in both autosomes and chromosome X, probably by mediating the merge of chromatin compartments . Plays a key role in chromosome X inactivation in females by promoting the spreading of heterochromatin .

Solicitud

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:500 - 1:2000
FI/CCI	1:50 - 1:100

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Descripción general

Descripción	Anticuerpo policlonal de conejo contra SMCHD1
Especificidad	Recognizes endogenous levels of SMCHD1 protein.
Tipo de anticuerpo	Anticuerpo primario
Inmunógeno	Recombinant fusion protein of human SMCHD1
Purificación	The antibody was purified by immunogen affinity chromatography.
Peso Molecular	Previsto: 85; Observado: 246 kD
Formulario/búfer	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Nombres alternativos	KIAA0650; Structural maintenance of chromosomes flexible hinge domain-containing protein 1; SMC hinge domain-containing protein 1
Símbolo genético	SMCHD1
Entrez Gene	23347 (humano); 74355 (ratón)
SwissProt	A6NHR9 (humano); Q6P5D8 (ratón)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Datos

Western blot analysis of SMCHD1 expression in SKOV3 (A), Jurkat (B), HepG2 (C), rat brain (D) whole cell lysates. (Predicted band size: 85; 215; 226 kD; Observed band size: 246 kD)

Immunofluorescent analysis of SMCHD1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Almacenamiento

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Nota

For Research Use Only. Not for use in diagnostic procedures.

Preguntas frecuentes

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

Nuevos productos

PE-labled Human IgG1 Mouse Monoclonal Antibody(ARA651)

PE-labled CD43 Mouse Monoclonal Antibody(ARA652)

Esfingosina 1-Kit ELISA de fosfato

Kit ELISA NGAL humano

Kit ELISA KIM-1 humano

HRP-conjugated Pig IgG (H&L) Rabbit Polyclonal Antibody