Plakophilin 3 Rabbit Polyclonal Antibody
MSDS
Key features and details
Rabbit polyclonal antibody to Plakophilin 3
- Objetivo:
- Fuente/Anfitrión:
- Reactividad:
- Clonalidad:
- Aplicaciones:
- Conjugación:
- Almacenamiento:
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Marca:
CAT.NO. : APA18113
US$ Por favor elige
US$ Por favor elige
Detalles del producto
Antecedentes
A component of desmosome cell-cell junctions which are required for positive regulation of cellular adhesion . Required for the localization of DSG2, DSP and PKP2 to mature desmosome junctions . May also play a role in the maintenance of DSG3 protein abundance in keratinocytes . Required for the formation of DSP-containing desmosome precursors in the cytoplasm during desmosome assembly . Also regulates the accumulation of CDH1 to mature desmosome junctions, via cAMP-dependent signaling and its interaction with activated RAP1A . Positively regulates the stabilization of PKP2 mRNA and therefore protein abundance, via its interaction with FXR1, may also regulate the protein abundance of DSP via the same mechanism . May also regulate the protein abundance of the desmosome component PKP1 .
Solicitud
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
WB | 1:500 - 1:1000 |
Descripción general
Descripción | Rabbit polyclonal antibody to Plakophilin 3 |
Especificidad | Recognizes endogenous levels of Plakophilin 3 protein. |
Tipo de anticuerpo | Anticuerpo primario |
Inmunógeno | KLH-conjugated synthetic peptide encompassing a sequence within the C-terminal region of human Plakophilin 3. The exact sequence is proprietary. |
Purificación | The antibody was purified by immunogen affinity chromatography. |
Peso Molecular | Predicted: 87 kD; Observed: 94 kD |
Formulario/búfer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Nombres alternativos | Placofilina-3 |
Símbolo genético | PKP3 |
Entrez Gene | 11187 (humano) |
SwissProt | Q9Y446(Humano) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Datos
Western blot analysis of Plakophilin 3 expression in A549 (A) whole cell lysates. (Predicted band size: 87 kD; Observed band size: 94 kD)
Almacenamiento
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Nota
For Research Use Only. Not for use in diagnostic procedures.
Preguntas frecuentes
What are the main types of research antibodies and how do they differ?
Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.
How can I tell if a research antibody is suitable for my experiment?
It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.
Can improper storage of research antibodies affect experimental results?
Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.
Why doesn’t the recommended dilution in the datasheet work well in my experiment?
The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.
What precautions should I take when using a newly purchased research antibody for the first time?
It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.
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