PCF11 Rabbit Polyclonal Antibody

MSDS

Key features and details

Rabbit polyclonal antibody to PCF11

Objetivo: PCF11
Source/Host: Conejo
Reactividad: Human, Mouse, Rat
Clonalidad: policlonal
Applications: WB
Conjugación: Sin conjugar
Almacenamiento: a-20°C

Marca:

CAT.NO. : APA16433

US$ Please choose

Tamaño:

50μL 100μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Antecedentes

Component of pre-mRNA cleavage complex II, which promotes transcription termination by RNA polymerase II.

Solicitud

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:500 - 1:2000

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Descripción general

Descripción	Rabbit polyclonal antibody to PCF11
Especificidad	Recognizes endogenous levels of PCF11 protein
Antibody Type	Primary antibody
Inmunógeno	Recombinant fusion protein of human PCF11. The exact sequence is proprietary.
Purificación	The antibody was purified by immunogen affinity chromatography.
Molecular Weight	Predicted: 173 kD; Observed: 180 kD
Formulario/búfer	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative Names	KIAA0824; Pre-mRNA cleavage complex 2 protein Pcf11; Pre-mRNA cleavage complex II protein Pcf11
Símbolo genético	PCF11
Entrez Gene	51585 (humano)
SwissProt	O94913(Human)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Datos

Western blot analysis of PCF11 expression in Jurkat (A), mouse testis (B) whole cell lysates. (Predicted band size: 173 kD; Observed band size: 180 kD)

Almacenamiento

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Nota

For Research Use Only. Not for use in diagnostic procedures.

Preguntas frecuentes

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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