Anticuerpo policlonal de conejo PAK1 (fosfo-T212)

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MSDS

PAK1 (Phospho-T212) Rabbit Polyclonal Antibody

Características y detalles clave

Anticuerpo policlonal de conejo contra PAK1 (Phospho-T212)

Objetivo: PAK1 (Fosfo-T212)
Fuente/Anfitrión: Conejo
Reactividad: Humano, ratón, rata
Clonalidad: policlonal
Aplicaciones: BM, CHI, FI/CCI
Conjugación: Sin conjugar
Almacenamiento: a-20°C

Marca:

CAT.NO. : APA09439

US$ Por favor elige

Tamaño:

50μL 100μL

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Detalles del producto

Antecedentes

Protein kinase involved in intracellular signaling pathways downstream of integrins and receptor-type kinases that plays an important role in cytoskeleton dynamics, in cell adhesion, migration, proliferation, apoptosis, mitosis, and in vesicle-mediated transport processes . Can directly phosphorylate BAD and protects cells against apoptosis . Activated by interaction with CDC42 and RAC1 . Functions as a GTPase effector that links the Rho-related GTPases CDC42 and RAC1 to the JNK MAP kinase pathway . Phosphorylates and activates MAP2K1, and thereby mediates activation of downstream MAP kinases . Involved in the reorganization of the actin cytoskeleton, actin stress fibers and of focal adhesion complexes .

Solicitud

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:500 - 1:1000
IHC	1:100 - 1:200
FI/CCI	1:100 - 1:500

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Descripción general

Descripción	Anticuerpo policlonal de conejo contra PAK1 (Phospho-T212)
Especificidad	Reconoce niveles endógenos de proteína PAK1 solo cuando está fosforilada en T212.
Tipo de anticuerpo	Anticuerpo primario
Inmunógeno	KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T212 of human PAK1 protein. The exact sequence is proprietary.
Purificación	El anticuerpo se purificó mediante cromatografía de afinidad de inmunógeno.
Peso Molecular	Previsto: 60 kD; Observado: 68 kD
Formulario/búfer	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Nombres alternativos	Serine/threonine-protein kinase PAK 1; Alpha-PAK; p21-activated kinase 1; PAK-1; p65-PAK
Símbolo genético	PAK1
Entrez Gene	5058 (humano); 29431 (Rata)
SwissProt	Q13153 (Humano); O88643(Ratón); P35465 (Rata)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Datos

Western blot analysis of PAK1 (Phospho-T212) expression in HCT116 (A) whole cell lysates. (Predicted band size: 60 kD; Observed band size: 68 kD)

Immunohistochemical analysis of PAK1 (Phospho-T212) staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of PAK1 (Phospho-T212) staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Almacenamiento

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Nota

Sólo para uso en investigación. No apto para uso diagnóstico, terapéutico, profiláctico o in vivo.

Preguntas frecuentes

¿Cuáles son los principales tipos de anticuerpos de investigación y en qué se diferencian?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

¿Cómo puedo saber si un anticuerpo de investigación es adecuado para mi experimento?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

¿Puede el almacenamiento inadecuado de anticuerpos de investigación afectar los resultados experimentales?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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