Anticuerpo policlonal de conejo NACA1 (fosfo-S43)

MSDS

NACA1 (Phospho-S43) Rabbit Polyclonal Antibody

Características y detalles clave

Rabbit polyclonal antibody to NACA1 (Phospho-S43)

Objetivo: NACA1 (Fosfo-S43)
Fuente/Anfitrión: Conejo
Reactividad: Humano, Ratón, Rata
Clonalidad: policlonal
Aplicaciones: WB, IHC
Conjugación: Sin conjugar
Almacenamiento: a-20°C

Marca:

CAT.NO. : APA10712

US$ Por favor elige

Tamaño:

50μL 100μL

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Detalles del producto

Antecedentes

Prevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). Binds to nascent polypeptide chains as they emerge from the ribosome and blocks their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. Also reduces the inherent affinity of ribosomes for protein translocation sites in the ER membrane (M sites). May act as a specific coactivator for JUN, binding to DNA and stabilizing the interaction of JUN homodimers with target gene promoters.

Solicitud

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:500 - 1:1000
IHC	1:50 - 1:100

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Descripción general

Descripción	Rabbit polyclonal antibody to NACA1 (Phospho-S43)
Especificidad	Recognizes endogenous levels of NACA1 protein only when phosphorylated at S43.
Tipo de anticuerpo	Anticuerpo primario
Inmunógeno	KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S43 of human NACA1 protein. The exact sequence is proprietary.
Purificación	The antibody was purified by immunogen affinity chromatography.
Peso Molecular	Previsto: 23 kD; Observado: 40 kD
Formulario/búfer	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Nombres alternativos	Nascent polypeptide-associated complex subunit alpha; NAC-alpha; Alpha-NAC; allergen Hom s 2
Símbolo genético	NACA
Entrez Gene	4666 (humano); 17938 (ratón)
SwissProt	Q13765 (Humano); Q60817 (ratón)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Datos

Western blot analysis of NACA1 (Phospho-S43) expression in HEK293T (A), Jurkat (B), NIH3T3 (C), CT26 (D), H9C2 (E), PC12 (F) whole cell lysates. (Predicted band size: 23 kD; Observed band size: 40 kD)

Immunohistochemical analysis of NACA1 (Phospho-S43) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Almacenamiento

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Nota

For Research Use Only. Not for use in diagnostic procedures.

Preguntas frecuentes

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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