MDL-1 Mouse Monoclonal Antibody(C2644)

Hoja de datos

MSDS

Key features and details

Mouse monoclonal to MDL-1

Objetivo: MDL-1
Fuente/Anfitrión: ratón
Reactividad: humano
Clonalidad: monoclonal
Aplicaciones: WB, FC
Conjugación: Sin conjugar
Almacenamiento: a-20°C

Marca:

CAT.NO. : AMA02256

US$ Please choose

Tamaño:

50μL 100μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Antecedentes

Functions as a positive regulator of osteoclastogenesis . Cell surface receptor that signals via TYROBP . Regulates inflammatory responses .

Solicitud

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:500 - 1:1000
FC	1:100 - 1:200

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Descripción general

Descripción	Mouse monoclonal to MDL-1
Especificidad	Recognizes endogenous levels of MDL-1 protein
Tipo de anticuerpo	Primary antibody
Inmunógeno	Recombinant fusion protein of human MDL-1 expressed in E. Coli
Purificación	This antibody is purified through a protein G column.
Molecular Weight	Predicted: 22 kD; Observed: 34 kD kD
Formulario/búfer	Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative Names	CLECSF5; MDL1; C-type lectin domain family 5 member A; C-type lectin superfamily member 5; Myeloid DAP12-associating lectin 1; MDL-1
Símbolo genético	CLEC5A
Entrez Gene	23601 (humano)
SwissProt	Q9NY25(Humano)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Datos

Western blot analysis of MDL-1 expression in THP1 (A) whole cell lysates. (Predicted band size: 22 kD; Observed band size: 34 kD kD)

Flow cytometric analysis of U937 cells using Anti-MDL-1 Antibody (green) and negative control (red).

Almacenamiento

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Nota

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

Preguntas frecuentes

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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