Anticuerpo policlonal de conejo LC3B

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Características y detalles clave

Anticuerpo policlonal de conejo contra LC3B

Objetivo: LC3B
Fuente/Anfitrión: Conejo
Reactividad: Humano, Ratón, Rata
Clonalidad: policlonal
Aplicaciones: BM, CHI, FI/CCI
Conjugación: Sin conjugar
Almacenamiento: a-20°C

Marca:

CAT.NO. : APA12463

US$ Por favor elige

Tamaño:

50μL 100μL

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Detalles del producto

Antecedentes

Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes) . Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production . In response to cellular stress and upon mitochondria fission, binds C-18 ceramides and anchors autophagolysosomes to outer mitochondrial membranes to eliminate damaged mitochondria . While LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation . Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway .

Solicitud

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:1000 - 1:2000
IHC	1:200 - 1:500
FI/CCI	1:100 - 1:200

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Descripción general

Descripción	Anticuerpo policlonal de conejo contra LC3B
Especificidad	Reconoce niveles endógenos de proteína LC3B.
Tipo de anticuerpo	Anticuerpo primario
Inmunógeno	Proteína recombinante correspondiente a LC3B humana.
Purificación	El anticuerpo se purificó mediante cromatografía de afinidad de inmunógeno.
Peso Molecular	Previsto: 14 kD; Observados: 14; 16kD
Formulario/búfer	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Nombres alternativos	MAP1ALC3; Microtubule-associated proteins 1A/1B light chain 3B; Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; MAP1 light chain 3-like protein 2; MAP1A/MAP1B light chain 3 B; MAP1A/MAP1B LC3 B; Microtubule-associated protein 1 light chain 3 beta
Símbolo genético	MAP1LC3B
Entrez Gene	81631 (humano); 67443 (ratón); 64862 (rata)
SwissProt	Q9GZQ8(Humano); Q9CQV6(Ratón); Q62625 (Rata)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Datos

Western blot analysis of LC3B expression in Hela (A), NIH3T3 (B), rat brain (C) whole cell lysates. (Predicted band size: 14 kD; Observed band size: 14; 16 kD)

Immunohistochemical analysis of LC3B staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of LC3B staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Almacenamiento

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Nota

Sólo para uso en investigación. No apto para uso en procedimientos de diagnóstico.

Preguntas frecuentes

¿Cuáles son los principales tipos de anticuerpos de investigación y en qué se diferencian?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

¿Cómo puedo saber si un anticuerpo de investigación es adecuado para mi experimento?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

¿Puede el almacenamiento inadecuado de anticuerpos de investigación afectar los resultados experimentales?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

¿Por qué la dilución recomendada en la hoja de datos no funciona bien en mi experimento?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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