JNK1/2/3 (Phospho-T183/Y185) Rabbit Monoclonal Antibody(C3224)

MSDS

JNK1/2/3 (Phospho-T183/Y185) Rabbit Monoclonal Antibody(C3224)

Características y detalles clave

Rabbit monoclonal antibody to JNK1/2/3 (Phospho-T183/Y185)

Objetivo: JNK1/2/3 (Fosfo-T183/Y185)
Fuente/Anfitrión: Conejo
Reactividad: humano, rata
Clonalidad: monoclonal
Aplicaciones: BM, IP
Conjugación: Sin conjugar
Almacenamiento: a-20°C

Marca:

CAT.NO. : AMA02836

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Tamaño:

50μL 100μL

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Detalles del producto

Antecedentes

Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as pro-inflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway signaling pathway . In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity . Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins .

Solicitud

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:500 - 1:1000
IP	1:10 - 1:50

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Descripción general

Descripción	Rabbit monoclonal antibody to JNK1/2/3 (Phospho-T183/Y185)
Especificidad	Recognizes endogenous levels of JNK1/2/3 protein only when phosphorylated at T183/Y185.
Tipo de anticuerpo	Anticuerpo primario
Inmunógeno	KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T183/Y185 of human JNK1/2/3 protein. The exact sequence is proprietary.
Purificación	The antibody was purified by immunogen affinity chromatography.
Peso Molecular	Previsto: 48 kD; Observado: 46,54 kD
Formulario/búfer	Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA.
Nombres alternativos	JNK1; PRKM8; SAPK1; SAPK1C; Mitogen-activated protein kinase 8; MAP kinase 8; MAPK 8; JNK-46; Stress-activated protein kinase 1c; SAPK1c; Stress-activated protein kinase JNK1; c-Jun N-terminal kinase 1
Símbolo genético	MAPK8
Entrez Gene	5599 (humano); 116554 (rata)
SwissProt	P45983 (Humano); P49185 (Rata)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Datos

Western blot analysis of JNK1/2/3 (Phospho-T183/Y185) expression in Hela (A), A549 (B), C6 (C) whole cell lysates. (Predicted band size: 48 kD; Observed band size: 46,54 kD)

Almacenamiento

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Nota

For Research Use Only. Not for use in diagnostic procedures.

Preguntas frecuentes

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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