HSV-tag Rabbit Polyclonal Antibody
Hoja de datos
Key features and details
Rabbit polyclonal antibody to HSV-tag
- Objetivo:
- Source/Host:
- Reactividad:
- Clonalidad:
- Applications:
- Conjugación:
- Almacenamiento:
-
Marca:
CAT.NO. : APA12342
US$ Please choose
US$ Please choose
Product Details
Antecedentes
The HSV-tag is an 11-amino-acid epitope (QPELAPEDPED) derived from herpes simplex virus glycoprotein D. It is commonly used as a fusion tag for detection of recombinant proteins by Western blot and immunoprecipitation. Anti-HSV antibodies offer high specificity with minimal background.
Solicitud
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
WB | 1:500 - 1:1000 |
IP | 1:10 - 1:100 |
Descripción general
Descripción | Rabbit polyclonal antibody to HSV-tag |
Especificidad | Recognizes the HSV-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. |
Antibody Type | Primary antibody,Tag |
Inmunógeno | KLH-conjugated synthetic peptide QPELAPEDPED. |
Purificación | The antibody was purified by immunogen affinity chromatography. |
Molecular Weight | N/A |
Formulario/búfer | Liquid in PBS, pH 7.3, 0.2% BSA, and 0.02% sodium azide. |
Alternative Names | |
Símbolo genético | |
Entrez Gene | |
SwissProt |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Datos
Western blot analysis of Anti-HSV-tag Antibody against HEK293T cells transfected with vector overexpressing HSV tag (A) and untransfected (B).
Almacenamiento
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Nota
For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.
Preguntas frecuentes
What are the main types of research antibodies and how do they differ?
Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.
How can I tell if a research antibody is suitable for my experiment?
It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.
Can improper storage of research antibodies affect experimental results?
Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.
Why doesn’t the recommended dilution in the datasheet work well in my experiment?
The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.
What precautions should I take when using a newly purchased research antibody for the first time?
It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.
Nuevos productos
