Anticuerpo monoclonal de ratón HSPA4 (C2752)

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Características y detalles clave

Monoclonal de ratón para HSPA4

Objetivo: HSPA4
Fuente/Anfitrión: ratón
Reactividad: humano, mono
Clonalidad: monoclonal
Aplicaciones: BM, IHC, IF/ICC, FC
Conjugación: Sin conjugar
Almacenamiento: a-20°C

Marca:

CAT.NO. : AMA02364

US$ Por favor elige

Tamaño:

50μL 100μL

Sendero, tamaño a granel o solicitudes personalizadas Por favor contáctenos

Detalles del producto

Antecedentes

Heat shock 70 kDa protein 4 (HSPA4), also known as HSP70RY, Heat shock 70-related protein APG-2, Heat shock protein family H member 2. Belongs to the heat shock protein 70 family Subunit: Interacts with TJP1/ZO-1 Subcellular location: Cytoplasm

Solicitud

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:500 - 1:1000
IHC	1:100 - 1:500
FI/CCI	1:100 - 1:500
FC	1:100 - 1:200

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Descripción general

Descripción	Monoclonal de ratón para HSPA4
Especificidad	Reconoce niveles endógenos de proteína HSPA4.
Tipo de anticuerpo	Anticuerpo primario
Inmunógeno	Recombinant fusion protein of human HSPA4 expressed in HEK293
Purificación	Este anticuerpo se purifica a través de una columna de proteína G.
Peso Molecular	Previsto: 94 kD; Observado: 100 kD kD
Formulario/búfer	Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Nombres alternativos	APG2; Heat shock 70 kDa protein 4; HSP70RY; Heat shock 70-related protein APG-2
Símbolo genético	HSPA4
Entrez Gene	3308 (humano)
SwissProt	P34932 (Humano)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Datos

Western blot analysis of HSPA4 expression in Hela (A), HepG2 (B), HEK293 (C), COS7 (D), A549 (E), Jurkat (F) whole cell lysates. (Predicted band size: 94 kD; Observed band size: 100 kD kD)

Immunohistochemical analysis of HSPA4 staining in human bladder cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of HSPA4 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with an AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AREX® Fluor 594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Flow cytometric analysis of Jurkat cells using Anti-HSPA4 Antibody (green) and negative control (red).

Almacenamiento

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Nota

For Research Use Only. Not for use in diagnostic procedures.

Preguntas frecuentes

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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