Anticuerpo policlonal de conejo GLUR1 (fosfo-S863)

MSDS

GLUR1 (Phospho-S863) Rabbit Polyclonal Antibody

Características y detalles clave

Anticuerpo policlonal de conejo contra GLUR1 (Phospho-S863)

Objetivo: GLUR1 (Fosfo-S863)
Fuente/Anfitrión: Conejo
Reactividad: Humano, Ratón, Rata
Clonalidad: policlonal
Aplicaciones: BM, CHI, FI/CCI
Conjugación: Sin conjugar
Almacenamiento: a-20°C

Marca:

CAT.NO. : APA11207

US$ Por favor elige

Tamaño:

50μL 100μL

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Detalles del producto

Antecedentes

Ionotropic glutamate receptor that functions as a ligand-gated cation channel, gated by L-glutamate and glutamatergic agonists such as alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA), quisqualic acid, and kainic acid , quisqualic acid, and kainic acid . L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse upon entry of monovalent and divalent cations such as sodium and calcium. The receptor then desensitizes rapidly and enters in a transient inactive state, characterized by the presence of bound agonist .

Solicitud

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:500 - 1:1000
IHC	1:50 - 1:200
FI/CCI	1:50 - 1:200

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Descripción general

Descripción	Anticuerpo policlonal de conejo contra GLUR1 (Phospho-S863)
Especificidad	Reconoce niveles endógenos de proteína GLUR1 solo cuando está fosforilada en S863.
Tipo de anticuerpo	Anticuerpo primario
Inmunógeno	KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S863 of human GLUR1 protein. The exact sequence is proprietary.
Purificación	El anticuerpo se purificó mediante cromatografía de afinidad de inmunógeno.
Peso Molecular	Predicho: 101 kD; Observado: 100 kD
Formulario/búfer	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Nombres alternativos	GLUH1; GLUR1; Glutamate receptor 1; GluR-1; AMPA-selective glutamate receptor 1; GluR-A; GluR-K1; Glutamate receptor ionotropic AMPA 1; GluA1
Símbolo genético	GRIA1
Entrez Gene	2890 (humano); 14799 (ratón); 50592 (Rata)
SwissProt	P42261 (Humano); P23818 (ratón); P19490 (Rata)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Datos

Western blot analysis of GLUR1 (Phospho-S863) expression in HCT116 (A), U87MG (B) whole cell lysates. (Predicted band size: 101 kD; Observed band size: 100 kD)

Immunohistochemical analysis of GLUR1 (Phospho-S863) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of GLUR1 (Phospho-S863) staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AREX® Fluor 594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Almacenamiento

Almacenar a 4°C por corto tiempo. Para almacenamiento a largo plazo, almacenar a -20°C, evitando ciclos de congelación/descongelación.

Nota

Sólo para uso en investigación. No apto para uso en procedimientos de diagnóstico.

Preguntas frecuentes

¿Cuáles son los principales tipos de anticuerpos de investigación y en qué se diferencian?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

¿Cómo puedo saber si un anticuerpo de investigación es adecuado para mi experimento?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

¿Puede el almacenamiento inadecuado de anticuerpos de investigación afectar los resultados experimentales?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

¿Por qué la dilución recomendada en la hoja de datos no funciona bien en mi experimento?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

¿Qué precauciones debo tomar al utilizar por primera vez un anticuerpo de investigación recién adquirido?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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