Drebrin Rabbit Polyclonal Antibody

MSDS

Características y detalles clave

Rabbit polyclonal antibody to Drebrin

Objetivo: Drebrin
Fuente/Anfitrión: Conejo
Reactividad: Humano, Ratón, Rata
Clonalidad: policlonal
Aplicaciones: BM, FI/CCI
Conjugación: Sin conjugar
Almacenamiento: a-20°C

Marca:

CAT.NO. : APA15413

US$ Por favor elige

Tamaño:

50μL 100μL

Trail, Bulk size or Custom requests Please contact us

Detalles del producto

Antecedentes

Actin cytoskeleton-organizing protein that plays a role in the formation of cell projections . Required for actin polymerization at immunological synapses (IS) and for the recruitment of the chemokine receptor CXCR4 to IS . Plays a role in dendritic spine morphogenesis and organization, including the localization of the dopamine receptor DRD1 to the dendritic spines . Involved in memory-related synaptic plasticity in the hippocampus .

Solicitud

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:500 - 1:2000
FI/CCI	1:50 - 1:200

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Descripción general

Descripción	Rabbit polyclonal antibody to Drebrin
Especificidad	Recognizes endogenous levels of Drebrin protein
Tipo de anticuerpo	Anticuerpo primario
Inmunógeno	Recombinant fusion protein of human Drebrin. The exact sequence is proprietary.
Purificación	The antibody was purified by immunogen affinity chromatography.
Peso Molecular	Previsto: 71; Observado: 110 kD
Formulario/búfer	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Nombres alternativos	D0S117E; Drebrin; Developmentally-regulated brain protein
Símbolo genético	DBN1
Entrez Gene	1627(Human); 56320(Mouse); 81653(Rat)
SwissProt	Q16643(Human); Q9QXS6(Mouse); Q07266(Rat)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Datos

Western blot analysis of Drebrin expression in HeLa (A), SKOV3 (B) whole cell lysates. (Predicted band size: 71; 76 kD; Observed band size: 110 kD)

Immunofluorescent analysis of Drebrin staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 488-conjugated secondary antibody (green) in PBS at room temperature in the dark.

Almacenamiento

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Nota

For Research Use Only. Not for use in diagnostic procedures.

Preguntas frecuentes

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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