CDX2 Mouse Monoclonal Antibody(C3351)

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MSDS

Características y detalles clave

Anticuerpo monoclonal de ratón contra CDX2

Objetivo: CDX2
Fuente/Anfitrión: ratón
Reactividad: Humano, ratón, mono
Clonalidad: monoclonal
Aplicaciones: BM, FI/CCI, IP
Conjugación: Sin conjugar
Almacenamiento: a-20°C

Marca:

CAT.NO. : AMA02963

US$ Por favor elige

Tamaño:

50μL 100μL

Trail, Bulk size or Custom requests Please contact us

Detalles del producto

Antecedentes

Transcription factor which regulates the transcription of multiple genes expressed in the intestinal epithelium . Binds to the promoter of the intestinal sucrase-isomaltase SI and activates SI transcription . Binds to the DNA sequence 5'-ATAAAAACTTAT-3' in the promoter region of VDR and activates VDR transcription . Binds to and activates transcription of LPH . Activates transcription of CLDN2 and intestinal mucin MUC2 . Binds to the 5'-AATTTTTTACAACACCT-3' DNA sequence in the promoter region of CA1 and activates CA1 transcription . Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine. Binds preferentially to methylated DNA .

Solicitud

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:500 - 1:1000
FI/CCI	1:50 - 1:100
IP	1:10 - 1:50

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Descripción general

Descripción	Anticuerpo monoclonal de ratón contra CDX2
Especificidad	Recognizes endogenous levels of CDX2 protein.
Tipo de anticuerpo	Anticuerpo primario
Inmunógeno	Purified recombinant human CDX2 protein fragments expressed in E.coli
Purificación	The antibody was purified by immunogen affinity chromatography.
Peso Molecular	Previsto: 34 kD; Observado: 34 kD
Formulario/búfer	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Nombres alternativos	CDX3; Homeobox protein CDX-2; CDX-3; Caudal-type homeobox protein 2
Símbolo genético	CDX2
Entrez Gene	1045 (humano); 12591 (ratón)
SwissProt	Q99626(Humano); P43241 (ratón)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Datos

Western blot analysis of CDX2 expression in Hela (A), Caco2 (B), C2C12 (C), SW480 (D), COS7 (E), HepG2 (F), NIH3T3 (G) whole cell lysates. (Predicted band size: 34 kD; Observed band size: 34 kD)

Immunofluorescent analysis of CDX2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark.

Almacenamiento

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Nota

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

Preguntas frecuentes

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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