CD8 Rabbit Monoclonal Antibody(ARB915)
Hoja de datos
Key features and details
- Objetivo:
- Identificación del clon:
- Fuente/Anfitrión:
- Reactividad:
- Aplicaciones:
- Dilución:
- Clonalidad:
- Almacenamiento:
-
Marca:
CAT.NO. : ARB6707
US$ Por favor elige
US$ Por favor elige
Tamaño:
Trail, Bulk size or Custom requests Please contact us
Detalles del producto
Antecedentes
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that serves as a coreceptor for TCR recognition of MHC class I-associated peptides and supports CTL activation by binding to the MHC, while making no direct contact with the peptide. CD8 is expressed on cytotoxic suppressor T cells. It is expressed as a heterodimer of CD8α and CD8β. CD8 is expressed on approximately one-third of peripheral blood T cells (the CD4-negative cells). CD8 has also been detected at a low level on some natural killer (NK) cells. In normal human tonsil, large numbers of CD8+ lymphocytes were present within the paracortex; occasionally positive cells were also identified within germinal centers and within the investing squamous epithelium. In other tissues, only lymphoid cells and cells of histiocyte lineage showed positive staining for CD8.
Solicitud
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
Solicitud | Relación de dilución |
IHC | 1:100 - 1:200 |
Descripción general
Predicted Molecular Wt | 26kDa |
Pureza | ProA affinity purified IgG |
Ubicación subcelular | membrana |
Identificación de Swissprot | P01732 |
inmunógeno | Synthetic peptide corresponding to CD8α residues within aa135-235 of CD8α. |
Búfer de almacenamiento | PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%. |
Método recomendado | Heat induced epitope retrieval with Tris-EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes. |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Datos
Immunohistochemical staining of human tonsil tissue using CD8 Rabbit Monoclonal Antibody (ARB915).
Almacenamiento
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Sólo para uso en investigación
For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.
Preguntas frecuentes
Are the pathology antibodies provided by AREX raw antibodies or ready-to-use solutions?
AREX Biosciences specializes in supplying high-quality IHC pathology raw antibodies (Raw Antibodies). We do not manufacture ready-to-use working solutions or IVD diagnostic reagents. We mainly provide concentrated raw materials to pathology reagent manufacturers and diagnostic platform companies to support product development and OEM production.
What development scenarios are pathology raw antibodies mainly used for?
Our raw antibodies are primarily used for the research, performance optimization, validation, and commercial-scale production of pathology IHC detection reagents. They are also suitable for companion diagnostics (CDx) projects and antibody screening.
How can I evaluate whether a raw antibody is suitable for our staining platform?
It is recommended to focus on specificity, sensitivity, background control, and performance on your target automated platforms (such as Roche Ventana, Leica Bond, Agilent Dako, etc.). We can provide internal test data for reference, but we recommend partners perform actual validation on their own platforms.
Can you provide samples for platform validation?
Yes. We can provide validation samples depending on the specific product. Some products may be provided free of charge, while others may involve a small sample fee. Handling fee and shipping fee will be charged separately. Please contact us for detailed confirmation.
How should raw antibodies be paired with detection systems?
AREX raw antibodies can be used with various polymer detection systems and ancillary reagents. We recommend optimization in combination with the ARExVisual® system or your existing detection platform to achieve better signal intensity and background control.
Nuevos productos
