BCL7A Rabbit Polyclonal Antibody

MSDS

Key features and details

Rabbit polyclonal antibody to BCL7A

Objetivo: BCL7A
Fuente/Anfitrión: Conejo
Reactividad: Humano, Ratón, Rata
Clonalidad: policlonal
Aplicaciones: WB
Conjugación: Sin conjugar
Almacenamiento: a-20°C

Marca:

CAT.NO. : APA11432

US$ Por favor elige

Tamaño:

50μL 100μL

Trail, Bulk size or Custom requests Please contact us

Detalles del producto

Antecedentes

This gene is directly involved, with Myc and IgH, in a three-way gene translocation in a Burkitt lymphoma cell line. As a result of the gene translocation, the N-terminal region of the gene product is disrupted, which is thought to be related to the pathogenesis of a subset of high-grade B cell non-Hodgkin lymphoma. The N-terminal segment involved in the translocation includes the region that shares a strong sequence similarity with those of BCL7B and BCL7C. Two transcript variants encoding different isoforms have been found for this gene.

Solicitud

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:500 - 1:1000

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Descripción general

Descripción	Rabbit polyclonal antibody to BCL7A
Especificidad	Recognizes endogenous levels of BCL7A protein.
Tipo de anticuerpo	Anticuerpo primario
Inmunógeno	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human BCL7A. The exact sequence is proprietary.
Purificación	The antibody was purified by immunogen affinity chromatography.
Peso Molecular	Predicted: 22 kD; Observed: 26 kD
Formulario/búfer	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Nombres alternativos	B-cell CLL/lymphoma 7 protein family member A
Símbolo genético	BCL7A
Entrez Gene	605(Human); 77045(Mouse)
SwissProt	Q4VC05(Human); Q9CXE2(Mouse)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Datos

Western blot analysis of BCL7A expression in rat ovary (A), mouse embryo (B), PC12 (C), CT26 (D), PC3 (E), A2780 (F), LOVO (G) whole cell lysates. (Predicted band size: 22 kD; Observed band size: 26 kD)

Almacenamiento

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Nota

For Research Use Only. Not for use in diagnostic procedures.

Preguntas frecuentes

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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