ATR Rabbit Polyclonal Antibody
MSDS
Key features and details
Rabbit polyclonal antibody to ATR
- Objetivo:
- Fuente/Anfitrión:
- Reactividad:
- Clonalidad:
- Aplicaciones:
- Conjugación:
- Almacenamiento:
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Marca:
CAT.NO. : APA15135
US$ Por favor elige
US$ Por favor elige
Detalles del producto
Antecedentes
Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor , ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor . Recognizes the substrate consensus sequence [ST]-Q . Phosphorylates BRCA1, CHEK1, MCM2, RAD17, RBBP8, RPA2, SMC1 and p53/TP53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis . Phosphorylates 'Ser-139' of histone variant H2AX at sites of DNA damage, thereby regulating DNA damage response mechanism . Required for FANCD2 ubiquitination .
Solicitud
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
WB | 1:500 - 1:2000 |
Descripción general
Descripción | Rabbit polyclonal antibody to ATR |
Especificidad | Recognizes endogenous levels of ATR protein |
Tipo de anticuerpo | Anticuerpo primario |
Inmunógeno | KLH-conjugated synthetic peptide of human ATR. The exact sequence is proprietary. |
Purificación | The antibody was purified by immunogen affinity chromatography. |
Peso Molecular | Predicted: 294; Observed: 301 kD |
Formulario/búfer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Nombres alternativos | FRP1; Serine/threonine-protein kinase ATR; Ataxia telangiectasia and Rad3-related protein; FRAP-related protein 1 |
Símbolo genético | ATR |
Entrez Gene | 545 (humano) |
SwissProt | Q13535(Human); Q9JKK8(Mouse) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Datos
Western blot analysis of ATR expression in NCIH460 (A) whole cell lysates. (Predicted band size: 294; 297; 301 kD; Observed band size: 301 kD)
Almacenamiento
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Nota
For Research Use Only. Not for use in diagnostic procedures.
Preguntas frecuentes
What are the main types of research antibodies and how do they differ?
Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.
How can I tell if a research antibody is suitable for my experiment?
It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.
Can improper storage of research antibodies affect experimental results?
Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.
Why doesn’t the recommended dilution in the datasheet work well in my experiment?
The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.
What precautions should I take when using a newly purchased research antibody for the first time?
It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.
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