DNMT3A Rabbit Monoclonal Antibody(C3239)
MSDS 
Key features and details
Rabbit monoclonal antibody to DNMT3A
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
CAT.NO. : AMA02851
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Product Details
Background
Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development . DNA methylation is coordinated with methylation of histones . It modifies DNA in a non-processive manner and also methylates non-CpG sites . May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1 . Plays a role in paternal and maternal imprinting . Required for methylation of most imprinted loci in germ cells . Acts as a transcriptional corepressor for ZBTB18 . Recruited to trimethylated 'Lys-36' of histone H3 (H3K36me3) sites . Can actively repress transcription through the recruitment of HDAC activity . Also has weak auto-methylation activity on Cys-710 in absence of DNA .
Application
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
WB | 1:500 - 1:1000 |
Overview
Description | Rabbit monoclonal antibody to DNMT3A |
Specificity | Recognizes endogenous levels of DNMT3A protein. |
Antibody Type | Primary antibody |
Imnunogen | A synthetic peptide of human Dnmt3a |
Purification | The antibody was purified by immunogen affinity chromatography. |
Molecular Weight | Predicted: 102 kD; Observed: 130 kD |
Form/Buffer | Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA. |
Alternative Names | DNA (cytosine-5)-methyltransferase 3A; Dnmt3a; DNA methyltransferase HsaIIIA; DNA MTase HsaIIIA; M.HsaIIIA |
Gene Symbol | DNMT3A |
Entrez Gene | 1788(Human) |
SwissProt | Q9Y6K1(Human) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Data
Western blot analysis of DNMT3A expression in Hela (A), A549 (B) whole cell lysates. (Predicted band size: 102 kD; Observed band size: 130 kD)
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Note
For Research Use Only. Not for use in diagnostic procedures.
FAQs
What are the main types of research antibodies and how do they differ?
Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.
How can I tell if a research antibody is suitable for my experiment?
It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.
Can improper storage of research antibodies affect experimental results?
Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.
Why doesn’t the recommended dilution in the datasheet work well in my experiment?
The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.
What precautions should I take when using a newly purchased research antibody for the first time?
It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.
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