AKT (Phospho-Y315) Polyklonaler Kaninchen-Antikörper

Datenblatt

Sicherheitsdatenblatt

AKT (Phospho-Y315) Rabbit Polyclonal Antibody

Hauptmerkmale und Details

Polyklonaler Kaninchen-Antikörper gegen AKT (Phospho-Y315)

Ziel: AKT (Phospho-Y315)
Quelle/Host: Kaninchen
Reaktivität: Mensch, Maus, Ratte, Rind, Huhn, Zebrafisch
Klonalität: Polyklonal
Anwendungen: WB, IHC, IF/ICC
Konjugation: Unkonjugiert
Lagerung: bei -20°C

Marke:

KAT.-NR. : APA06636

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Größe:

50μL 100 μL

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Produktdetails

Hintergrund

AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis . This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates . Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported . AKT is responsible of the regulation of glucose uptake by mediating insulin-induced translocation of the SLC2A4/GLUT4 glucose transporter to the cell surface .

Bewerbung

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:500 - 1:1000
IHC	1:100 - 1:200
IF/ICC	1:100 - 1:500

*Ergebnisse sind probenspezifisch. Bitte beziehen Sie sich als Referenz auf Ihre lokalen Testbedingungen und Testparameter.

Übersicht

Beschreibung	Polyklonaler Kaninchen-Antikörper gegen AKT (Phospho-Y315)
Spezifität	Erkennt endogene Mengen an AKT-Protein nur, wenn es an Y315 phosphoryliert ist.
Antikörpertyp	Primärer Antikörper
Immunogen	KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y315 of human AKT protein. The exact sequence is proprietary.
Reinigung	Der Antikörper wurde durch Immunogenaffinitätschromatographie gereinigt.
Molekulargewicht	Voraussichtlich: 55 kD; Beobachtet: 60 kD
Form/Puffer	Flüssigkeit aus 0,42 % Kaliumphosphat, 0,87 % Natriumchlorid, pH 7,3, 30 % Glycerin und 0,01 % Natriumazid.
Alternative Namen	AKT1; PKB; RAC; RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha; AKT2; RAC-beta serine/threonine-protein kinase; Protein kinase Akt-2; Protein kinase B beta; PKB beta; RAC-PK-beta; AKT3; PKBG; RAC-gamma serine/threonine-protein kinase; Protein kinase Akt-3; Protein kinase B gamma; PKB gamma; RAC-PK-gamma; STK-2
Gensymbol	AKT1; AKT2; AKT3
Entrez Gene	207; 208; 10000 (Mensch); 11651; 11652; 23797(Maus); 24185; 25233; 29414(Ratte)
SwissProt	P31749; P31751; Q9Y243 (Mensch); P31750; Q60823; Q9WUA6(Maus); P47196; P47197; Q63484(Ratte)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Klonnummer, Reaktivität, Quelle/Host und Klonalität finden Sie oben im Abschnitt Produktname und Hauptfunktionen.

Daten

Western blot analysis of AKT (Phospho-Y315) expression in U87MG (A) whole cell lysates. (Predicted band size: 55 kD; Observed band size: 60 kD)

Immunohistochemical analysis of AKT (Phospho-Y315) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of AKT (Phospho-Y315) staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Direct ELISA antibody dose-response curve using Anti-AKT (Phospho-Y315) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.

Lagerung

Kurzfristig bei 4°C lagern. Bei längerer Lagerung bei -20 °C lagern und Einfrier-/Auftauzyklen vermeiden.

Hinweis

Nur für Forschungszwecke. Nicht für diagnostische, therapeutische, prophylaktische oder in vivo-Anwendungen.

FAQs

Was sind die wichtigsten Arten von Forschungsantikörpern und wie unterscheiden sie sich?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

Wie kann ich feststellen, ob ein Forschungsantikörper für mein Experiment geeignet ist?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Kann eine unsachgemäße Lagerung von Forschungsantikörpern die Versuchsergebnisse beeinflussen?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Warum funktioniert die im Datenblatt empfohlene Verdünnung in meinem Experiment nicht?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

Welche Vorsichtsmaßnahmen sollte ich treffen, wenn ich einen neu erworbenen Forschungsantikörper zum ersten Mal verwende?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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