Cytokeratin 6A/B/C Rabbit Monoclonal Antibody(ARA810)

Datasheet

MSDS

Cytokeratin 6A/B/C Rabbit Monoclonal Antibody(ARA810)

Key features and details

Target: Cytokeratin 6A/B/C
Source/Host: Rabbit
Reactivity: Human, Mouse, Rat
Clonality: Monoclonal
Applications: WB,IHC,IP
Conjugation: Unconjugated
Storage: at-20°C

Brand:

CAT.NO. : ARA6597

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Size:

100μL

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Product Details

Background

Epidermis-specific type I keratin involved in wound healing. Involved in the activation of follicular keratinocytes after wounding, while it does not play a major role in keratinocyte proliferation or migration. Participates in the regulation of epithelial migration by inhibiting the activity of SRC during wound repair.

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:5000-1:10000
IHC	1:50-1:100
IP	1:50

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Description	Rabbit Monoclonal Antibody to Cytokeratin 6A/B/C
Antibody Type	Primary antibody
Predicted MW	60kDa
Immunogen	A synthetic peptide corresponding to residues on the C-terminus of human Cytokeratin 6A/B/C was used as an immunogen.
Purification	ProA affinity purified IgG
Form/Buffer	PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.85%.
Alternative Names	KRT6E; Keratin, type II cytoskeletal 6C; Cytokeratin-6C; CK-6C; Cytokeratin-6E; CK-6E; Keratin K6h; Keratin-6C; K6C; Type-II keratin Kb12
Gene Symbol	KRT6C
Entrez Gene	286887(Human)
Swissprot	P48668

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

All lanes: Anti-Cytokeratin 6A/B/C antibody at 1:10,000 dilution
Predicted MW: 60 kDa
Observed MW: 60 kDa
Lane 1: A431
Lane 2: Mu Skin
Lane 3: Rat Skin
Lysates at 10 µg per lane
2nd Ab:
GAR HRP(H+L) 1:5,000
Exposure: 20s

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervix cancer tissue labelling Cytokeratin 6A/B/C with ARA810 at 1:50. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.

Cytokeratin 6A/B/C was immunoprecipitated from 0.4mg of A431 whole cell lysate with ARA810 at 1:50 dilution.
2nd Ab:
GAR HRP for IP 1:500
Lane 1: ARA810 IP in A431 whole cell lysate
Lane 2: PBS instead of ARA810 in A431 whole cell lysate
Lane 3: A431 whole cell lysate, 10 µg (input)
Exposure: 120s

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Note

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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