CD74 Mouse Monoclonal Antibody(C2611)
Datasheet 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
Product Details
WB | 1:500 - 1:1000 |
IF/ICC | 1:100 - 1:500 |
FC | 1:100 - 1:200 |
Description | Mouse monoclonal to CD74 |
Specificity | Recognizes endogenous levels of CD74 protein |
Antibody Type | Primary antibody |
Imnunogen | Recombinant fusion protein of human CD74 expressed in E. Coli |
Purification | This antibody is purified through a protein G column. |
Molecular Weight | Predicted: 34 kD; Observed: 32 kD kD |
Form/Buffer | Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Names | DHLAG; HLA class II histocompatibility antigen gamma chain; HLA-DR antigens-associated invariant chain; Ia antigen-associated invariant chain; Ii; p33; CD74 |
Gene Symbol | CD74 |
Entrez Gene | 972(Human) |
SwissProt | P04233(Human) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of CD74 expression in Raji (A) whole cell lysates. (Predicted band size: 34 kD; Observed band size: 32 kD kD)
Immunofluorescent analysis of CD74 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with an AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AREX® Fluor 594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Flow cytometric analysis of Jurkat cells using Anti-CD74 Antibody (green) and negative control (red).
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