CaMK2 alpha/beta/delta (Phospho-T305) Rabbit Polyclonal Antibody

Datasheet

MSDS

CaMK2 alpha/beta/delta (Phospho-T305) Rabbit Polyclonal Antibody

Key features and details

Rabbit polyclonal antibody to CaMK2 alpha/beta/delta (Phospho-T305)

Target: CaMK2 alpha/beta/delta (Phospho-T305)
Source/Host: Rabbit
Reactivity: Human,Mouse,Rat,Bovine,Chicken,Pig,Rabbit,Zebrafish
Clonality: Polyclonal
Applications: WB, IHC
Conjugation: Unconjugated
Storage: at-20°C

Brand:

CAT.NO. : APA10904

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Size:

50μL 100μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in various processes, such as synaptic plasticity, neurotransmitter release and long-term potentiation /calmodulin-binding and autophosphorylation, and is involved in various processes, such as synaptic plasticity, neurotransmitter release and long-term potentiation . Member of the NMDAR signaling complex in excitatory synapses, it regulates NMDAR-dependent potentiation of the AMPAR and therefore excitatory synaptic transmission . Regulates dendritic spine development . Also regulates the migration of developing neurons . Phosphorylates the transcription factor FOXO3 to activate its transcriptional activity .

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:500 - 1:1000
IHC	1:50 - 1:100

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Description	Rabbit polyclonal antibody to CaMK2 alpha/beta/delta (Phospho-T305)
Specificity	Recognizes endogenous levels of CaMK2 alpha/beta/delta protein only when phosphorylated at T305.
Antibody Type	Primary antibody
Imnunogen	KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T305 of human CaMK2 alpha/beta/delta protein. The exact sequence is proprietary.
Purification	The antibody was purified by immunogen affinity chromatography.
Molecular Weight	Predicted: 54; Observed: 50 kD
Form/Buffer	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative Names	CAMK2A; CAMKA; KIAA0968; Calcium/calmodulin-dependent protein kinase type II subunit alpha; CaM kinase II subunit alpha; CaMK-II subunit alpha; CAMK2B; CAM2; CAMK2; CAMKB; Calcium/calmodulin-dependent protein kinase type II subunit beta; CaM kinase II subunit beta; CaMK-II subunit beta; CAMK2D; CAMKD; Calcium/calmodulin-dependent protein kinase type II subunit delta; CaM kinase II subunit delta; CaMK-II subunit delta
Gene Symbol	CAMK2A
Entrez Gene	815; 816; 817(Human); 12322; 12323; 108058(Mouse); 25400; 24245; 24246(Rat)
SwissProt	Q9UQM7; Q13554; Q13557(Human); P11798; P28652; Q6PHZ2(Mouse); P11275; P08413; P15791(Rat)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Western blot analysis of CaMK2 alpha/beta/delta (Phospho-T305) expression in HEK293T (A), U87MG (B), A549 (C), rat brain (D) whole cell lysates. (Predicted band size: 54; 72; 56 kD; Observed band size: 50 kD)

Immunohistochemical analysis of CaMK2 alpha/beta/delta (Phospho-T305) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Note

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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