B7-H3 Rabbit Monoclonal Antibody(ARM598)
Datasheet 
Key features and details
- Target:
- Clone ID:
- Source/Host:
- Reactivity:
- Applications:
- Dilution:
- Clonality:
- Storage:
-
Brand:
CAT.NO. : ARM6889
US$ Please choose
US$ Please choose
Size:
Trail, Bulk size or Custom requests Please contact us
Product Details
Background
B7 homolog 3 (B7-H3), also known as CD276, belongs the B7 immunoglobulin superfamily of cell surface ligands that regulate T cell activation and immune responses. Soluble B7-H3 binds a putative receptor on activated T-cells that is distinct from CD28, CTLA-4, ICOS and PD-1. B7-H3 is expressed by antigen presenting cells, activated T cells, and a few normal tissues, including placenta and prostate. Expression of B7-H3 is also seen in several cancer types, such as prostate, breast, colon, lung, and gastric cancers.
Application
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
Application | IHC |
Dilution Ratio | 1:50 - 1:200 |
Overview
Antibody Type | Primary antibodies |
Isotype | IgG |
Positive Control | Tonsil |
Localization | Membranous |
Form/Buffer | Tris Buffer, pH 7.3 - 7.7, with 1% BSA and <0.1% Sodium Azide |
Purification | Purified |
Conjugation | Unconjugated |
Alternative Names | CD276 |
Gene Symbol | CD276 |
Entrez Gene | 80381 |
Uniprot | Q5ZPR3 |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Data
Immunohistochemical staining of human tonsil tissue using B7-H3 Rabbit Monoclonal Antibody (ARM598).
Research Use Only
For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
FAQs
Are the pathology antibodies provided by AREX raw antibodies or ready-to-use solutions?
AREX Biosciences specializes in supplying high-quality IHC pathology raw antibodies (Raw Antibodies). We do not manufacture ready-to-use working solutions or IVD diagnostic reagents. We mainly provide concentrated raw materials to pathology reagent manufacturers and diagnostic platform companies to support product development and OEM production.
What development scenarios are pathology raw antibodies mainly used for?
Our raw antibodies are primarily used for the research, performance optimization, validation, and commercial-scale production of pathology IHC detection reagents. They are also suitable for companion diagnostics (CDx) projects and antibody screening.
How can I evaluate whether a raw antibody is suitable for our staining platform?
It is recommended to focus on specificity, sensitivity, background control, and performance on your target automated platforms (such as Roche Ventana, Leica Bond, Agilent Dako, etc.). We can provide internal test data for reference, but we recommend partners perform actual validation on their own platforms.
Can you provide samples for platform validation?
Yes. We can provide validation samples depending on the specific product. Some products may be provided free of charge, while others may involve a small sample fee. Handling fee and shipping fee will be charged separately. Please contact us for detailed confirmation.
How should raw antibodies be paired with detection systems?
AREX raw antibodies can be used with various polymer detection systems and ancillary reagents. We recommend optimization in combination with the ARExVisual® system or your existing detection platform to achieve better signal intensity and background control.
New Products
