Anti-Serotype 8b Fowl Adenovirus (FAdV-8b) Fiber Protein Mouse Monoclonal Antibody (5F10)
Datasheet 
Key features and details
- Target:
- Host:
- Reactivity:
- Clonality:
- Application:
- Storage:
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Brand:
CAT.NO. : AXA0029
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Product Details
Background
Serotype 8b Fowl Adenovirus (FAdV-8b) Fiber Protein is a key structural protein of FAdV-8b, a member of the Aviadenovirus genus. It is a protruding fiber-like structure on the viral capsid, playing a crucial role in viral host cell recognition and entry by binding to specific receptors on the surface of host cells. This protein exhibits distinct immunogenic properties, capable of inducing the host to produce specific antibodies, and serves as an important target for the development of FAdV-8b detection methods and subunit vaccines. Its amino acid sequence and functional domains have characteristics that distinguish it from other FAdV serotypes, which are closely related to its host tropism and pathogenicity.
Application
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
Application | Dilution Ratio |
IFA | 1:500-1:1000 |
WB | 1:2000-1:5000 |
Overview
Description | Mouse Monoclonal antibody to Serotype 8b Fowl Adenovirus (FAdV-8b) Fiber Protein |
Antibody Type | Primary antibody |
Isotype | IgG |
Immunogen | Prokaryotic expression product GST-Fiber of FAdV-8b Fiber gene |
Form/Buffer | PBS, 20% Glycerol; preservative: 0.05% Sodium Azide |
Alternative Names | FAdV-8b Fiber, Fowl adenovirus 8b fiber protein, FAdV serotype 8b fiber |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Data
The WB results showed that the monoclonal antibodies 4D9 and 5F10 can effectively react with LMH cells overexpressing FAdV-8b Fiber and LMH cells infected with FAdV-8b.
The IFA results showed that the monoclonal antibodies 4D9 and 5F10 can effectively react with LMH cells overexpressing FAdV-8b Fiber and LMH cells infected with FAdV-8b.
Specific detection using WB showed that the monoclonal antibody 5F10 can effectively recognize the Fiber proteins of FAdV-7 and FAdV-8b.
Reference
1. Lu H, Shao H, Chen H, et al. Identification of novel B cell epitopes in the fiber protein of serotype 8 Fowl adenovirus. AMB Express. 2019;9(1):172. doi:10.1186/s13568-019-0895-1
2. Lu H, Guo Y, Xu Z, et al. Fiber-1 of serotype 4 fowl adenovirus mediates superinfection resistance against serotype 8b fowl adenovirus. Front Microbiol. 2022;13:1086383. doi:10.3389/fmicb.2022.1086383
2. Lu H, Guo Y, Xu Z, et al. Fiber-1 of serotype 4 fowl adenovirus mediates superinfection resistance against serotype 8b fowl adenovirus. Front Microbiol. 2022;13:1086383. doi:10.3389/fmicb.2022.1086383
Storage
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Note
For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.
FAQs
What are the main types of research antibodies and how do they differ?
Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.
How can I tell if a research antibody is suitable for my experiment?
It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.
Can improper storage of research antibodies affect experimental results?
Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.
Why doesn’t the recommended dilution in the datasheet work well in my experiment?
The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.
What precautions should I take when using a newly purchased research antibody for the first time?
It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.
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