Anti-Serotype 8b Fowl Adenovirus (FAdV-8b) Fiber Protein Mouse Monoclonal Antibody (5F10)
Key features and details
- Clone:
- Host:
- Species Reactivity:
- Application:
- Immunogen :
- Clone Type:
- Format:
- Storage:
-
Brand:
CAT.NO. : AXA0029
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Size:
Trail, Bulk size or Custom requests Please contact us
Product Details
Overview
|
Host |
Mouse |
|
Antibody Isotype |
IgG |
|
Clonality |
Monoclonal |
|
Use |
IFA, WB |
|
Reactivity |
FAdV-7 Fiber Protein, FAdV-8b Fiber Protein |
|
Appearance |
Liquid |
|
Storage Buffer |
PBS, 20% Glycerol; preservative: 0.05% Sodium Azide |
|
Storage Condition |
Store at -20°C, valid for 1 year, avoid repeated freeze-thaw cycles; can be stored at 4°C for 1-2 weeks. |
|
Concentration |
1 mg/mL |
|
Immunogen |
Prokaryotic expression product GST-Fiber of FAdV-8b Fiber gene |
Application
|
Application |
Recommended Dilution Ratio |
|
IFA |
1:1000 |
|
WB |
1:2000 - 1:5000 |
Data
The results of WB and IFA showed that the monoclonal antibodies 4D9 and 5F10 can effectively react with LMH cells overexpressing FAdV-8b Fiber and LMH cells infected with FAdV-8b.
Specific detection using WB showed that the monoclonal antibody 5F10 can effectively recognize the Fiber proteins of FAdV-7 and FAdV-8b; 4D9 can only effectively recognize the Fiber protein of FAdV-8b.
Reference
1. Lu H, Shao H, Chen H, et al. Identification of novel B cell epitopes in the fiber protein of serotype 8 Fowl adenovirus. AMB Express. 2019;9(1):172. doi:10.1186/s13568-019-0895-1
2. Lu H, Guo Y, Xu Z, et al. Fiber-1 of serotype 4 fowl adenovirus mediates superinfection resistance against serotype 8b fowl adenovirus. Front Microbiol. 2022;13:1086383. doi:10.3389/fmicb.2022.1086383
2. Lu H, Guo Y, Xu Z, et al. Fiber-1 of serotype 4 fowl adenovirus mediates superinfection resistance against serotype 8b fowl adenovirus. Front Microbiol. 2022;13:1086383. doi:10.3389/fmicb.2022.1086383
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Intended use
For Research Use Only. Not for use in diagnostic procedures.
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