Anti-Serotype 4 Fowl Adenovirus (FAdV-4) Fiber-2 Protein Mouse Monoclonal Antibody (1D7)

Key features and details

Product: Anti-Serotype 4 Fowl Adenovirus (FAdV-4) Fiber-2 Protein Mouse Monoclonal Antibody (1D7)
Clone: 1D7
Host: Mouse
Species Reactivity: FAdV-4 Fiber-2 Protein
Application: IFA,IP
Immunogen : GST-Fiber-2
Clone Type: Monoclonal
Storage: -20.0°C

Brand:

CAT.NO. : AXA0027

US$ Please choose

Size:

100μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

Serotype 4 Fowl Adenovirus (FAdV-4) Fiber-2 Protein is a key structural protein of Fowl Adenovirus Serotype 4, a non-enveloped double-stranded DNA virus with an icosahedral capsid (70-90 nm in diameter) that protrudes one or two fiber proteins. It plays a critical role in viral entry by recognizing and binding to specific receptors on host cell surfaces. It exhibits strong immunogenicity, capable of inducing neutralizing antibodies in the host—subunit vaccines based on this protein can provide 100% protection for SPF chickens against FAdV-4 challenge when the immunizing antigen dose ranges from 25-100 μg per chicken. It has significant applications in FAdV-4 detection and vaccine development: the indirect ELISA antibody detection method established with this protein is sensitive, specific and accurate for quantifying antibody levels in immunized chickens; its subunit vaccines offer advantages like high safety and simple preparation processes compared to traditional attenuated or inactivated vaccines, making it a promising direction for FAdV-4 vaccine research.

Overview

Host	Mouse
Antibody Isotype	IgG
Clonality	Monoclonal
Use	IFA, IP
Reactivity	FAdV-4 Fiber-2 Protein
Appearance	Liquid
Storage Buffer	PBS, 20% Glycerol; preservative: 0.05% Sodium Azide
Storage Condition	Store at -20°C, valid for 1 year, avoid repeated freeze-thaw cycles; can be stored at 4°C for 1-2 weeks.
Concentration	1 mg/mL
Immunogen	Prokaryotic expression product GST-Fiber-2 of FAdV-4 Fiber-2 gene

Application

Application	Recommended Dilution Ratio
IFA	1:500-1:1000
WB	1:2000-1:5000
IP	1:200-1:1000

*The optimal dilution ratio/concentration should be determined by researchers.

Data

A: LMH cells infected with FAdV-4, B: LMH cells infected with FAdV-5, C: LMH cells infected with FAdV-6,
D: LMH cells infected with FAdV-7a, E: LMH cells infected with FAdV-8a, F: LMH cells infected with FAdV-8b,
G: LMH cells infected with FAdV-9, H: LMH cells infected with FAdV-10, I: Uninfected LMH cells
IFA results showed that monoclonal antibodies 1C9 and 3C2 can effectively recognize LMH cells infected with FAdV-4 and FAdV-10;
Monoclonal antibody 1D7 can effectively recognize LMH cells infected with FAdV-4; and the three strains of monoclonal antibodies have good specificity.

A: LMH cells infected with FAdV-4, B: LMH cells infected with FAdV-10, C: Uninfected LMH cells
The WB results showed that the monoclonal antibody 1C9 can effectively recognize the Fiber-2 protein of FAdV-4 and FAdV-10

A: LMH cells infected with FAdV-4, B: LMH cells infected with FAdV-10, C: Uninfected LMH cells
The IP results showed that the monoclonal antibody 1D7 can effectively immunoprecipitate the Fiber-2 protein of FAdV-4

A: LMH cells infected with FAdV-4, B: LMH cells infected with FAdV-10, C: Uninfected LMH cells
The IP results showed that the monoclonal antibody 3C2 could effectively immunoprecipitate the Fiber-2 proteins of FAdV-4 and FAdV-10

Reference

1. Wang P, Zhang J, Wang W, et al. A novel monoclonal antibody efficiently blocks the infection of serotype 4 fowl adenovirus by targeting fiber-2. Vet Res. 2018;49(1):29. doi:10.1186/s13567-018-0525-y
2. Xie S, Zhang J, Chen H, et al. Development of colloidal gold-based test strip for rapid detection of serotype 4 fowl adenovirus. J Virol Methods. 2021;296:114231. doi:10.1016/j.jviromet.2021.114231
3. Xie Q, Wang W, Kan Q, et al. FAdV-4 without Fiber-2 Is a Highly Attenuated and Protective Vaccine Candidate. Microbiol Spectr. 2022;10(1):e0143621. doi:10.1128/spectrum.01436-21
4. Xie Q, Cao S, Zhang W, et al. A novel fiber-2-edited live attenuated vaccine candidate against the highly pathogenic serotype 4 fowl adenovirus. Vet Res. 2021;52(1):35. Published 2021 Feb 27. doi:10.1186/s13567-021-00907-z
5. Xie Q, Wang W, Li L, et al. Domain in Fiber-2 interacted with KPNA3/4 significantly affects the replication and pathogenicity of the highly pathogenic FAdV-4. Virulence. 2021;12(1):754-765. doi:10.1080/21505594.2021.1888458
6. Mu Y, Xie Q, Wang W, et al. A Novel Fiber-1-Edited and Highly Attenuated Recombinant Serotype 4 Fowl Adenovirus Confers Efficient Protection Against Lethal Challenge. Front Vet Sci. 2021;8:759418. doi:10.3389/fvets.2021.759418
7. Wang W, Liu Q, Li T, et al. Fiber-1, Not Fiber-2, Directly Mediates the Infection of the Pathogenic Serotype 4 Fowl Adenovirus via Its Shaft and Knob Domains. J Virol. 2020;94(17):e00954-20. doi:10.1128/JVI.00954-20

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Intended use

For Research Use Only. Not for use in diagnostic procedures.

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