Anti-Serotype 4 Fowl Adenovirus (FAdV-4) Fiber-2 Protein Mouse Monoclonal Antibody (1D7)
Key features and details
- Clone:
- Host:
- Species Reactivity:
- Application:
- Immunogen :
- Clone Type:
- Format:
- Storage:
-
Brand:
CAT.NO. : AXA0027
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Size:
Trail, Bulk size or Custom requests Please contact us
Product Details
Overview
|
Host |
Mouse |
|
Antibody Isotype |
IgG |
|
Clonality |
Monoclonal |
|
Use |
IFA, IP |
|
Reactivity |
FAdV-4 Fiber-2 Protein |
|
Appearance |
Liquid |
|
Storage Buffer |
PBS, 20% Glycerol; preservative: 0.05% Sodium Azide |
|
Storage Condition |
Store at -20°C, valid for 1 year, avoid repeated freeze-thaw cycles; can be stored at 4°C for 1-2 weeks. |
|
Concentration |
1 mg/mL |
|
Immunogen |
Prokaryotic expression product GST-Fiber-2 of FAdV-4 Fiber-2 gene |
Application
|
Application |
Recommended Dilution Ratio |
|
IFA |
1:1000 |
|
WB |
1:2000 - 1:5000 |
|
IP |
1:1000 |
Data
A: LMH cells infected with FAdV-4, B: LMH cells infected with FAdV-5, C: LMH cells infected with FAdV-6,
D: LMH cells infected with FAdV-7a, E: LMH cells infected with FAdV-8a, F: LMH cells infected with FAdV-8b,
G: LMH cells infected with FAdV-9, H: LMH cells infected with FAdV-10, I: Uninfected LMH cells
IFA results showed that monoclonal antibodies 1C9 and 3C2 can effectively recognize LMH cells infected with FAdV-4 and FAdV-10;
Monoclonal antibody 1D7 can effectively recognize LMH cells infected with FAdV-4; and the three strains of monoclonal antibodies have good specificity.
A: LMH cells infected with FAdV-4, B: LMH cells infected with FAdV-10, C: Uninfected LMH cells
The WB results showed that the monoclonal antibody 1C9 can effectively recognize the Fiber-2 protein of FAdV-4 and FAdV-10
A: LMH cells infected with FAdV-4, B: LMH cells infected with FAdV-10, C: Uninfected LMH cells
The IP results showed that the monoclonal antibody 1D7 can effectively immunoprecipitate the Fiber-2 protein of FAdV-4
A: LMH cells infected with FAdV-4, B: LMH cells infected with FAdV-10, C: Uninfected LMH cells
The IP results showed that the monoclonal antibody 3C2 could effectively immunoprecipitate the Fiber-2 proteins of FAdV-4 and FAdV-10
Reference
1. Wang P, Zhang J, Wang W, et al. A novel monoclonal antibody efficiently blocks the infection of serotype 4 fowl adenovirus by targeting fiber-2. Vet Res. 2018;49(1):29. doi:10.1186/s13567-018-0525-y
2. Xie S, Zhang J, Chen H, et al. Development of colloidal gold-based test strip for rapid detection of serotype 4 fowl adenovirus. J Virol Methods. 2021;296:114231. doi:10.1016/j.jviromet.2021.114231
3. Xie Q, Wang W, Kan Q, et al. FAdV-4 without Fiber-2 Is a Highly Attenuated and Protective Vaccine Candidate. Microbiol Spectr. 2022;10(1):e0143621. doi:10.1128/spectrum.01436-21
4. Xie Q, Cao S, Zhang W, et al. A novel fiber-2-edited live attenuated vaccine candidate against the highly pathogenic serotype 4 fowl adenovirus. Vet Res. 2021;52(1):35. Published 2021 Feb 27. doi:10.1186/s13567-021-00907-z
5. Xie Q, Wang W, Li L, et al. Domain in Fiber-2 interacted with KPNA3/4 significantly affects the replication and pathogenicity of the highly pathogenic FAdV-4. Virulence. 2021;12(1):754-765. doi:10.1080/21505594.2021.1888458
6. Mu Y, Xie Q, Wang W, et al. A Novel Fiber-1-Edited and Highly Attenuated Recombinant Serotype 4 Fowl Adenovirus Confers Efficient Protection Against Lethal Challenge. Front Vet Sci. 2021;8:759418. doi:10.3389/fvets.2021.759418
7. Wang W, Liu Q, Li T, et al. Fiber-1, Not Fiber-2, Directly Mediates the Infection of the Pathogenic Serotype 4 Fowl Adenovirus via Its Shaft and Knob Domains. J Virol. 2020;94(17):e00954-20. doi:10.1128/JVI.00954-20
2. Xie S, Zhang J, Chen H, et al. Development of colloidal gold-based test strip for rapid detection of serotype 4 fowl adenovirus. J Virol Methods. 2021;296:114231. doi:10.1016/j.jviromet.2021.114231
3. Xie Q, Wang W, Kan Q, et al. FAdV-4 without Fiber-2 Is a Highly Attenuated and Protective Vaccine Candidate. Microbiol Spectr. 2022;10(1):e0143621. doi:10.1128/spectrum.01436-21
4. Xie Q, Cao S, Zhang W, et al. A novel fiber-2-edited live attenuated vaccine candidate against the highly pathogenic serotype 4 fowl adenovirus. Vet Res. 2021;52(1):35. Published 2021 Feb 27. doi:10.1186/s13567-021-00907-z
5. Xie Q, Wang W, Li L, et al. Domain in Fiber-2 interacted with KPNA3/4 significantly affects the replication and pathogenicity of the highly pathogenic FAdV-4. Virulence. 2021;12(1):754-765. doi:10.1080/21505594.2021.1888458
6. Mu Y, Xie Q, Wang W, et al. A Novel Fiber-1-Edited and Highly Attenuated Recombinant Serotype 4 Fowl Adenovirus Confers Efficient Protection Against Lethal Challenge. Front Vet Sci. 2021;8:759418. doi:10.3389/fvets.2021.759418
7. Wang W, Liu Q, Li T, et al. Fiber-1, Not Fiber-2, Directly Mediates the Infection of the Pathogenic Serotype 4 Fowl Adenovirus via Its Shaft and Knob Domains. J Virol. 2020;94(17):e00954-20. doi:10.1128/JVI.00954-20
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Intended use
For Research Use Only. Not for use in diagnostic procedures.
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