Anti-Serotype 4 Fowl Adenovirus (FAdV-4) Fiber-1 Protein Mouse Monoclonal Antibody (3B5)
Key features and details
- Product:
- Clone:
- Host:
- Species Reactivity:
- Application:
- Immunogen :
- Clone Type:
- Storage:
-
Brand:
CAT.NO. : AXA0024
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Size:
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Product Details
Background
Serotype 4 Fowl Adenovirus (FAdV-4) Fiber-1 Protein is a key structural glycoprotein protruding from the viral capsid surface that plays a pivotal role in viral infection by mediating the recognition and attachment to specific receptors on host chicken cells it is a major immunogenic component of FAdV-4 contributing significantly to the induction of protective immune responses in infected hosts including the production of neutralizing antibodies its unique sequence and structural features distinguish it from Fiber proteins of other FAdV serotypes making it a specific target for serological diagnosis and epidemiological surveillance of FAdV-4 infections it also serves as a core antigen for the development of subunit vaccines and diagnostic reagents aimed at preventing and controlling hydropericardium-hepatitis syndrome caused by FAdV-4.
Overview
|
Host |
Mouse |
|
Antibody Isotype |
IgG |
|
Clonality |
Monoclonal |
|
Use |
IFA, IP |
|
Reactivity |
FAdV-C (FAdV-4/10) Fiber-1 Protein |
|
Appearance |
Liquid |
|
Storage Buffer |
PBS, 20% Glycerol; preservative: 0.05% Sodium Azide |
|
Storage Condition |
Store at -20°C, valid for 1 year, avoid repeated freeze-thaw cycles; can be stored at 4°C for 1-2 weeks. |
|
Concentration |
1 mg/mL |
|
Immunogen |
Prokaryotic expression product GST-Fiber-1 of FAdV-4 Fiber-1 gene |
Application
|
Application |
Recommended Dilution Ratio |
|
IFA |
1:200-1:1000 |
|
IP |
1:200-1:1000 |
*The optimal dilution ratio/concentration should be determined by researchers.
Data
A: LMH cells infected with FAdV-4, B: LMH cells infected with FAdV-8b
C: LMH cells transfected with pcDNA3.1-Fiber-1, D: LMH cells transfected with pcDNA3.1
The IFA results showed that the monoclonal antibody 3B5 can effectively recognize LMH cells infected with FAdV-4 and LMH cells overexpressing FAdV-4 Fiber-1
IP: 3B5 mAb
WB: Anti-Fiber-1 mouse-derived
A: LMH cells transfected with pcDNA3.1-Fiber-1, B: LMH cell control transfected with pcDNA3.1, C: LMH cells infected with FAdV-4-WT, D: Uninfected LMH cell control
The IP results showed that the monoclonal antibody 3B5 can effectively immunoprecipitate the FAdV-4 Fiber-1 protein
Reference
1. Shao H, Lu Y, Wang W, et al. Two novel monoclonal antibodies against fiber-1 protein of FAdV-4 and their application in detection of FAdV-4/10. BMC Vet Res. 2019;15(1):232. doi:10.1186/s12917-019-1987-5
2. Xie Q, Wang W, Kan Q, et al. FAdV-4 without Fiber-2 Is a Highly Attenuated and Protective Vaccine Candidate. Microbiol Spectr. 2022;10(1):e0143621. doi:10.1128/spectrum.01436-21
3. Xie Q, Cao S, Zhang W, et al. A novel fiber-2-edited live attenuated vaccine candidate against the highly pathogenic serotype 4 fowl adenovirus. Vet Res. 2021;52(1):35. doi:10.1186/s13567-021-00907-z
4. Xie Q, Wang W, Li L, et al. Domain in Fiber-2 interacted with KPNA3/4 significantly affects the replication and pathogenicity of the highly pathogenic FAdV-4. Virulence. 2021;12(1):754-765. doi:10.1080/21505594.2021.1888458
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Intended use
For Research Use Only. Not for use in diagnostic procedures.
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