ACTN3 Rabbit Polyclonal Antibody
MSDS 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
-
Brand:
Product Details
WB | 1:500 - 1:1000 |
IF/ICC | 1:100 - 1:500 |
Description | Rabbit polyclonal antibody to ACTN3 |
Specificity | Recognizes endogenous levels of ACTN3 protein. |
Antibody Type | Primary antibody |
Imnunogen | KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human ACTN3. The exact sequence is proprietary. |
Purification | The antibody was purified by immunogen affinity chromatography. |
Molecular Weight | Predicted: 103 kD; Observed: 100 kD |
Form/Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Names | Alpha-actinin-3; Alpha-actinin skeletal muscle isoform 3; F-actin cross-linking protein |
Gene Symbol | ACTN3 |
Entrez Gene | 89(Human); 11474(Mouse) |
SwissProt | Q08043(Human); O88990(Mouse) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of ACTN3 expression in HeLa (A), mouse muscle (B), rat muscle (C) whole cell lysates. (Predicted band size: 103 kD; Observed band size: 100 kD)
Immunofluorescent analysis of ACTN3 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
FAQs
New Products
