PYCRL Rabbit Polyclonal Antibody

MSDS'ler

Key features and details

Rabbit polyclonal antibody to PYCRL

Hedef: PYCRL
Kaynak/Ana Bilgisayar: Tavşan
Reaktivite: İnsan
Klonalite: poliklonal
Uygulamalar: WB
Konjugasyon: konjuge olmayan
Depolama: 20°C'de

Marka:

KAT.NO. : APA18820

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Boyut:

50μL 100μL

Trail, Bulk size or Custom requests Please contact us

Ürün Detayları

Arkaplan

Oxidoreductase that catalyzes the last step in proline biosynthesis, which corresponds to the reduction of pyrroline-5-carboxylate (P5C) to L-proline using NAD(P)H to L-proline using NAD(P)H . Proline is synthesized from either glutamate or ornithine; both are converted to P5C, and then to proline via pyrroline-5-carboxylate reductases (PYCRs) . PYCR3 is exclusively linked to the biosynthesis of proline from ornithine .

Başvuru

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:500 - 1:1000

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Genel Bakış

Açıklama	Rabbit polyclonal antibody to PYCRL
özgüllük	Recognizes endogenous levels of PYCRL protein.
Antikor Tipi	Birincil antikor
İmmünojen	KLH-conjugated synthetic peptide encompassing a sequence within the C-terminal region of human PYCRL. The exact sequence is proprietary.
Arıtma	The antibody was purified by immunogen affinity chromatography.
Molekül Ağırlığı	Predicted: 28 kD; Observed: 32 kD
Form/Arabellek	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternatif İsimler	Pyrroline-5-carboxylate reductase 3; P5C reductase 3; P5CR 3; Pyrroline-5-carboxylate reductase-like protein
Gen Sembolü	PYCRL
Entrez Geni	65263(İnsan)
İsviçreProt	Q53H96(İnsan)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Veri

Western blot analysis of PYCRL expression in A549 (A), Hela (B), KG1 (C) whole cell lysates. (Predicted band size: 28 kD; Observed band size: 32 kD)

Depolama

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Not

For Research Use Only. Not for use in diagnostic procedures.

SSS

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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