GMEB1 Rabbit Monoclonal Antibody(C1998)
Veri Sayfası
Temel özellikler ve ayrıntılar
Recombinant rabbit monoclonal antibody to GMEB1
- Hedef:
- Kaynak/Ana Bilgisayar:
- Reaktivite:
- Klonalite:
- Uygulamalar:
- Konjugasyon:
- Depolama:
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Marka:
KAT.NO. : AMA01610
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Ürün Detayları
Arkaplan
Acts as a DNA-binding transcriptional regulator involved in modulating the expression of genes responsive to glucocorticoid signaling . Specifically binds AT-rich DNA motifs known as glucocorticoid modulatory elements (GMEs), repressing or enhancing transcription depending on cellular context . Forms heterodimeric complexes with GMEB2, which enhances its DNA-binding specificity and transcriptional activity. This complex plays a critical role in the repression of glucocorticoid receptor (GR/NR3C1) transcriptional activity, acting as a negative modulator of glucocorticoid signaling . Regulates dopamine-enriched midbrain genes, including tyrosine hydroxylase/TH and the dopamine transporter SLC6A3, essential for dopamine signaling . Also binds the transferrin receptor promoter.
Başvuru
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
WB | 1:500 - 1:1000 |
IP | 1:10 - 1:50 |
Genel Bakış
Açıklama | Recombinant rabbit monoclonal antibody to GMEB1 |
özgüllük | Recognizes endogenous levels of GMEB1 protein |
Antikor Tipi | Primary antibody, Recombinant |
İmmünojen | Recombinant fusion protein of human GMEB1. The exact sequence is proprietary. |
Arıtma | The antibody was purified by immunogen affinity chromatography. |
Molekül Ağırlığı | Predicted: 62 kD; Observed: 63 kD |
Form/Arabellek | Liquid in PBS, pH 7.3, 50% glycerol, 0.05% BSA, and 0.05% Proclin300. |
Alternatif İsimler | Glucocorticoid modulatory element-binding protein 1; GMEB-1; DNA-binding protein p96PIF; Parvovirus initiation factor p96; PIF p96 |
Gen Sembolü | GMEB1 |
Entrez Geni | 10691(Human); 56809(Mouse); 500558(Rat) |
İsviçreProt | Q9Y692(Human); Q9JL60(Mouse); Q9QUZ8(Rat) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Veri
Western blot analysis of GMEB1 expression in HeLa (A) whole cell lysates. (Predicted band size: 62 kD; Observed band size: 63 kD)
Depolama
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Not
For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.
SSS
What are the main types of research antibodies and how do they differ?
Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.
How can I tell if a research antibody is suitable for my experiment?
It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.
Can improper storage of research antibodies affect experimental results?
Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.
Why doesn’t the recommended dilution in the datasheet work well in my experiment?
The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.
What precautions should I take when using a newly purchased research antibody for the first time?
It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.
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