Nestin Rabbit Monoclonal Antibody(ARB503)
Datablad
Key features and details
- Mål:
- Klon-ID:
- Källa/värd:
- Reaktivitet:
- Applikationer:
- Spädning:
- Klonalitet:
- Lagring:
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Märke:
CAT.NO. : ARB6795
US$ Please choose
US$ Please choose
Storlek:
Trail, Bulk size or Custom requests Please contact us
Produktdetaljer
Bakgrund
Nestin is an intermediate filament family member protein that is structurally related to the neurofilament proteins. It is highly expressed in the developing brain, where it may help to regulate cell structure and intracellular processes required for neural cell division and migration.Research studies have shown that expression of nestin is also found in cells from various nervous system tumors, including gliomas, neuroblastomas, astrocytomas, and it is generally accepted as a marker for neural cancer stem cells.Nestin expression is significantly increased in melanoma and correlated with more advanced stages of the disease.
Ansökan
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
Ansökan | Spädningsförhållande |
IHC | 1:100 - 1:200 |
Översikt
Predicted Molecular Wt | 177 kDa |
Renhet | ProA affinity purified IgG |
Subcellular location | Cytoplasma |
Swissprot ID | P48681 |
Immunogen | Synthetic peptide. |
Förvaringsbuffert | PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05% |
Recommended Method | Heat induced epitope retrieval with Tris-EDTA buffer (pH 9.0), primary antibody incubate at RT (18℃-25℃) for 30 minutes |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Data
Immunohistochemical staining of human malignant melanoma tissue using Nestin Rabbit Monoclonal Antibody(ARB503)
Förvaring
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Research Use Only
For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.
Vanliga frågor
Are the pathology antibodies provided by AREX raw antibodies or ready-to-use solutions?
AREX Biosciences specializes in supplying high-quality IHC pathology raw antibodies (Raw Antibodies). We do not manufacture ready-to-use working solutions or IVD diagnostic reagents. We mainly provide concentrated raw materials to pathology reagent manufacturers and diagnostic platform companies to support product development and OEM production.
What development scenarios are pathology raw antibodies mainly used for?
Our raw antibodies are primarily used for the research, performance optimization, validation, and commercial-scale production of pathology IHC detection reagents. They are also suitable for companion diagnostics (CDx) projects and antibody screening.
How can I evaluate whether a raw antibody is suitable for our staining platform?
It is recommended to focus on specificity, sensitivity, background control, and performance on your target automated platforms (such as Roche Ventana, Leica Bond, Agilent Dako, etc.). We can provide internal test data for reference, but we recommend partners perform actual validation on their own platforms.
Can you provide samples for platform validation?
Yes. We can provide validation samples depending on the specific product. Some products may be provided free of charge, while others may involve a small sample fee. Handling fee and shipping fee will be charged separately. Please contact us for detailed confirmation.
How should raw antibodies be paired with detection systems?
AREX raw antibodies can be used with various polymer detection systems and ancillary reagents. We recommend optimization in combination with the ARExVisual® system or your existing detection platform to achieve better signal intensity and background control.
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