CD79a Mouse Monoclonal Antibody(C2358)

Datablad

MSDS

Nyckelfunktioner och detaljer

Mouse monoclonal antibody to CD79a

Mål: CD79a
Källa/värd: Mus
Reaktivitet: Människan
Klonalitet: Monoklonal
Applikationer: IHC
Konjugering: Okonjugerad
Lagring: vid-20°C

Märke:

KAT.NR. : AMA01970

US$ Välj

Storlek:

50 μL 100 μL

Trail, Bulk size or Custom requests Please contact us

Produktdetaljer

Bakgrund

Required in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells. Stimulates SYK autophosphorylation and activation. Binds to BLNK, bringing BLNK into proximity with SYK and allowing SYK to phosphorylate BLNK. Also interacts with and increases activity of some Src-family tyrosine kinases. Represses BCR signaling during development of immature B-cells.

Ansökan

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

IHC	1:100 - 1:300

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Översikt

Beskrivning	Mouse monoclonal antibody to CD79a
Specificitet	Recognizes endogenous levels of CD79a protein.
Antikroppstyp	Primär antikropp
Imnunogen	KLH-conjugated synthetic peptide encompassing a sequence within human CD79a. The exact sequence is proprietary.
Rening	The antibody was purified by immunogen affinity chromatography.
Molekylvikt	N/A
Form/buffert	Mouse IgG2b. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.
Alternativa namn	IGA; MB1; B-cell antigen receptor complex-associated protein alpha chain; Ig-alpha; MB-1 membrane glycoprotein; Membrane-bound immunoglobulin-associated protein; Surface IgM-associated protein; CD79a
Gensymbol	CD79A
Entrez Gene	973 (människa)
SwissProt	P11912 (människa)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Immunohistochemical analysis of CD79a staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Förvaring

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Obs

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

Vanliga frågor

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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