Anticorpo Policlonal de Coelho UCP3

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Principais recursos e detalhes

Anticorpo policlonal de coelho para UCP3

Alvo: UCP3
Fonte/Hospedeiro: Coelho
Reatividade: Humano, Rato, Rato, Cachorro, Porco
Clonalidade: Policlonal
Aplicações: BM, IF/ICC
Conjugação: Não conjugado
Armazenamento: a-20°C

Marca:

GATO.NÃO. : APA09797

US$ Por favor escolha

Tamanho:

50μL 100μL

Trail, Bulk size or Custom requests Please contact us

Detalhes do produto

Plano de fundo

Putative transmembrane transporter that plays a role in mitochondrial metabolism via an as yet unclear mechanism . Originally, this mitochondrial protein was thought to act as a proton transmembrane transporter from the mitochondrial intermembrane space into the matrix, causing proton leaks through the inner mitochondrial membrane, thereby uncoupling mitochondrial membrane potential generation from ATP synthesis . However, this function is controversial and uncoupling may not be the function, or at least not the main function, but rather a consequence of more conventional metabolite transporter activity .

Aplicação

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:500 - 1:1000
SE/ICC	1:50 - 1:200

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Visão geral

Descrição	Anticorpo policlonal de coelho para UCP3
Especificidade	Recognizes endogenous levels of UCP3 protein.
Tipo de anticorpo	Anticorpo primário
Imunogênio	KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human UCP3. The exact sequence is proprietary.
Purificação	The antibody was purified by immunogen affinity chromatography.
Peso molecular	Previsto: 34 kD; Observado: 35 kD
Formulário/Buffer	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Nomes alternativos	SLC25A9; Mitochondrial uncoupling protein 3; UCP 3; Solute carrier family 25 member 9
Símbolo Genético	UCP3
Gene Entrez	7352(Humano); 22229(Rato); 25708 (Rato)
SwissProt	P55916(Human); P56501(Mouse); P56499(Rat)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Dados

Western blot analysis of UCP3 expression in mouse muscle (A), rat kidney (B) whole cell lysates. (Predicted band size: 34 kD; Observed band size: 35 kD)

Immunofluorescent analysis of UCP3 staining in MDAMB231 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AREX® Fluor 594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Armazenamento

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Nota

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

Perguntas frequentes

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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