Laminin alpha 5 Rabbit Polyclonal Antibody

Ficha técnica

FISPQ

Laminin alpha 5 Rabbit Polyclonal Antibody

Principais recursos e detalhes

Rabbit polyclonal antibody to Laminin alpha 5

Alvo: Laminina alfa 5
Fonte/Hospedeiro: Coelho
Reatividade: Humano, Rato
Clonalidade: Policlonal
Aplicações: WB, IHC, IP
Conjugação: Não conjugado
Armazenamento: a-20°C

Marca:

GATO.NÃO. : APA07199

US$ Por favor escolha

Tamanho:

50μL 100μL

Trail, Bulk size or Custom requests Please contact us

Detalhes do produto

Plano de fundo

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Plays a role in the regulation of skeletogenesis, through a mechanism that involves integrin-mediated signaling and PTK2B/PYK2 .

Aplicação

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:500 - 1:1000
IHQ	1:100 - 1:200
IP	1:10 - 1:100

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Visão geral

Descrição	Rabbit polyclonal antibody to Laminin alpha 5
Especificidade	Recognizes endogenous levels of Laminin alpha 5 protein.
Tipo de anticorpo	Anticorpo primário
Imunogênio	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Laminin alpha 5. The exact sequence is proprietary.
Purificação	The antibody was purified by immunogen affinity chromatography.
Peso molecular	Predicted: 399 kD; Observed: 400 kD
Formulário/Buffer	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Nomes alternativos	KIAA0533; KIAA1907; Laminin subunit alpha-5; Laminin-10 subunit alpha; Laminin-11 subunit alpha; Laminin-15 subunit alpha
Símbolo Genético	LAMA5
Gene Entrez	3911(Humano); 16776 (Rato)
SwissProt	O15230(Humano); Q61001 (Rato)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Dados

Western blot analysis of Laminin alpha 5 expression in JAR (A), A431 (B), RAW264.7 (C) whole cell lysates. (Predicted band size: 399 kD; Observed band size: 400 kD)

Immunohistochemical analysis of Laminin alpha 5 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Armazenamento

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Nota

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

Perguntas frequentes

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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